# MECHANISMS OF MINOR H ANTIGEN GVHD

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 1987 · $3,851

## Abstract

A murine model has been developed of graft-versus-host disease (GVHD) due
to minor histocompatibility antigens (minor HA) in two strain combinations
selected for H-2 identity and for mutual nonreactivity in MLC:  C57BL/6-LP
(H-2b combination) and B10.D2/nSn-BALB/c (H-2d combination).  This
model possesses many characteristics in common with human GVHD, including
acute and chronic forms and has been used to systematically study the
immune mechanisms that produce GVHD in response to minor HA.  A new assay
of the proliferative response to minor HA has been developed to study the
role of non-CTL in the pathogenesis of minor HA-GVHD.  Congenic strains of
mice developed between C57BL/10, LP, and 129 mice have been used to
demonstrate that multiple minor H antigenic differences must exist between
donor and recipient strains for GVH to develop.  Studies are in progress to
evaluate the immunoregulation of cellular responses to minor HA in mice
with transplants.

Attempts to study the proliferative response of spleen cells from mice with
GVHD to recipient strain and third party antigens led to the demonstration
that these spleen cells were unable to respond to foreign antigens in mixed
lymphocyte culture.  Further studies demonstrated that mice with minor
antigen GVHD have an acquired form of severe combined immunodeficiency and
are unable to develop either cell-mediated or humoral immunity to specific
antigens.  The specific mechanism of this immunodeficiency is under
investigation using both in vivo and in vitro techniques.  Using adoptive
transfer methods, we have been able to transfer minor antigen GVHD to
secondary recipients.  The cells that transfer GVHD are Thy-1+ and Lyt-2+.
The ceIls that initiate GVHD have been further defined:  the Lyt phenotype
varies with the specific strain combination tested.  (LB)

## Key facts

- **NIH application ID:** 3170626
- **Project number:** 5R01CA032757-06
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **SAM.gov UEI:** HD1WMN6945W6
- **Principal Investigator:** BRIAN L HAMILTON
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $3,851
- **Award type:** 5
- **Project period:** 1982-07-01 → 1987-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170626

## Citation

> US National Institutes of Health, RePORTER application 3170626, MECHANISMS OF MINOR H ANTIGEN GVHD (5R01CA032757-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170626. Licensed CC0.

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