# HYPERTHERMIA, RADIATION, MEMBRANE STRUCTURE AND FUNCTION

> **NIH NIH R01** · STANFORD UNIVERSITY · 1991 · $192,536

## Abstract

This research is intended to provide information on changes that occur in
plasma membranes of mammalian cells during and after exposure of such cells
to elevated temperatures.  The hypothesis to be tested is that these
changes are, at least in part, responsible for cell death at hyperthermic
temperature and, perhaps also, for the induction of thermotolerance.  To
this end, four sets of experiments are performed:  1) The cytotoxic action
of detergents and phospholipid analogs is tested at elevated temperatures.
These compounds have a wide range of lipophilicities and critical micelle
concentrations.  2) The cytotoxic action of stearic acid doxyl probes is
used to test for transmembrane locations of heat-sensitive sites.  3) The
response of ion channels to electrical stimulation is examined at
hyperthermic temperatures.  These experiments involve both ion-specific and
nonspecific electrodes.  Operation of the individual ion channels is
characterized as a function of temperature.  4) Epithelial cells are used
to identify the role that cell-to-cell contact (as distinct from cell
density) plays in modifying response of cells to heat.

Overall, these sets of experiments are intended to locate heat sensitive
sites within the plasma membrane, identify specific proteins there that are
affected by heat, and test how functional membrane modifications affect
heat response.

## Key facts

- **NIH application ID:** 3170664
- **Project number:** 2R01CA032827-09
- **Recipient organization:** STANFORD UNIVERSITY
- **SAM.gov UEI:** HJD6G4D6TJY5
- **Principal Investigator:** GEORGE M HAHN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $192,536
- **Award type:** 2
- **Project period:** 1982-08-01 → 1993-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170664

## Citation

> US National Institutes of Health, RePORTER application 3170664, HYPERTHERMIA, RADIATION, MEMBRANE STRUCTURE AND FUNCTION (2R01CA032827-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170664. Licensed CC0.

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