# DIFFERENTIATION AND FUNCTION OF HUMAN MONOCYTES

> **NIH NIH R01** · WISTAR INSTITUTE · 1990 · $213,792

## Abstract

Lymphocytes and hematopoietic cells of the myelomonocytic lineage
reciprocally interact during differentiation and in their roles as
regulatory and effector cells of adaptive and non-adaptive immunity.
Lymphocyte products, or lymphokines, are particularly important in
regulating activation and function of phagocytic cells of the
myelomonocytic lineage during immune response or inflammation.  Lymphokines
also have a regulatory role on proliferation, differentiation, and
functional activity of cells of the myelomonocytic lineage at different
stages of differentiation, from the precursor to the terminally
differentiated cells.  Although these effects have been determined in vitro
or in vivo in pathologic situations, it is likely that the effects observed
in pathology are an exaggeration of the physiologic role played by
lymphocytes and their products in regulation of the homeostasis of the
hematopoietic system.

The long range objective of this project is to identify and investigate the
mechanisms of interaction between lymphocytes and myelomonocytic cells.
Part of our studies are performed using in vitro established human myeloid
cell lines as a model system of differentiation in which regulation by
lymphokines of differentiation-associated markers is analyzed at phenotypic
and molecular levels.  The study will be focused on the investigation of
the molecular mechanisms by which tumor necrosis factor and interferon-
gamma act synergistically in inducing monocytic differentiation in immature
myeloid cells.  The modulating effects of other lymphokines will also by
analyzed.  Among other markers of differentiation, the maturation of the
NADPH oxidase system and respiratory burst capabilities during normal and
lymphokine-induced myelomonocytic cell differentiation will be
investigated.  The information obtained in these model systems is then
applied to the study of normal and leukemic cells, in order to understand
how differentiation and maturation are regulated in physiologic conditions
and their alteration in malignant myelomonocytic cells.

## Key facts

- **NIH application ID:** 3170773
- **Project number:** 2R01CA032898-09
- **Recipient organization:** WISTAR INSTITUTE
- **SAM.gov UEI:** DW1XZMGNFBL4
- **Principal Investigator:** DANIELA SANTOLI
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $213,792
- **Award type:** 2
- **Project period:** 1982-08-01 → 1995-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170773

## Citation

> US National Institutes of Health, RePORTER application 3170773, DIFFERENTIATION AND FUNCTION OF HUMAN MONOCYTES (2R01CA032898-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170773. Licensed CC0.

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