# DIFFERENTIATION AND FUNCTION OF HUMAN MONOCYTES

> **NIH NIH R01** · WISTAR INSTITUTE · 1989 · $170,978

## Abstract

The overall objective of this research is to characterize the process of
differentiation of human monocytes-macrophages from the precursor cells in
bone marrow and in the peripheral blood to the macrophage stage with
particular emphasis on the variation of antigenic expression and on the
development of specific functions.  The experimental models used will
include:  (1) established lines of human hematopoietic cells and primary
cultures of leukemia cells induced to differentiate in vitro by different
chemical or biological inducers; and (2) in vitro growth and
differentiation of normal precursor and stem cells from bone marrow and
peripheral blood.  The general aims are to determine whether an imbalance
in the expression of surface markers during induced differentiation of
these cells exists.  One of the results of the first year of this project
was the finding that immune interferon induces both the receptor for the Fc
fragment of monomeric IgG and differentiation aIong the monocytic lineage
in myeloid cells.  The current studies are mostly focused to analyze these
effects of immune interferon and to compare them with those of other
chemical and naturally occurring inducers.  Immune interferon is effective
both on immature and differentiated cells of the myelomonocytic lineage.
In immature myeloid cells, both normal and leukemic, immune interferon
induces differentiation, with acquisition of monocytic markers and
functions and loss of proliferative ability.  On differentiated cells
(granulocytes, monocytes, macrophages), immune interferon acts as an
activation factor, inducing increased expression of HLA-DR and Fc receptor,
enhanced phagocytosis, antibody-dependent celI-mediated cytotoxicity,
lysosomal enzyme release, and increased oxidative metabolism.  (MB)

## Key facts

- **NIH application ID:** 3170777
- **Project number:** 5R01CA032898-08
- **Recipient organization:** WISTAR INSTITUTE
- **SAM.gov UEI:** DW1XZMGNFBL4
- **Principal Investigator:** DANIELA SANTOLI
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $170,978
- **Award type:** 5
- **Project period:** 1982-08-01 → 1990-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170777

## Citation

> US National Institutes of Health, RePORTER application 3170777, DIFFERENTIATION AND FUNCTION OF HUMAN MONOCYTES (5R01CA032898-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170777. Licensed CC0.

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