# TYPE 12 ADENOVIRUS TRANSFORMATION - DEFECTIVE MUTANTS

> **NIH NIH R01** · CARNEGIE-MELLON UNIVERSITY · 1987 · $143,355

## Abstract

The overall aim of this project is to use genetic and molecular approaches
to define the functions that the various type 12 E1 proteins play in
transforming mouse and rat cells in vitro, and to understand the basis for
the striking differences in viral oncogenicity between this serotype and
non-oncogenic type 5.  Our specific aims over the next three years are:  1)
To extend genetic and biochemical characterization of the host-range (hr)
mutants of type 12 which we have recently isolated.  2) To isolate and
characterize conditional transformation-defective mutants of this
serotype.  3) To isolate and characterize site-specific mutants of type 12,
particularly those which alter E1A mRNA splice junctions.  4) To isolate
type 12 transformants of A549 cells.  5) To analyze the transformation
process in mouse kidney cells, and determine if epithelial and fibroblastic
subpopulations are transformed at different frequencies.  6) Extend our
study of viral oncogenicity and cellular malignancy by testing the ability
of hr mutants to induce tumors and tumor specific transplantation
immunity.  7) Using type 12 hr mutants, develop a complementing system for
isolation and growth of non-conditionally defective mutants.

## Key facts

- **NIH application ID:** 3170822
- **Project number:** 5R01CA032940-06
- **Recipient organization:** CARNEGIE-MELLON UNIVERSITY
- **SAM.gov UEI:** U3NKNFLNQ613
- **Principal Investigator:** JAMES F WILLIAMS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $143,355
- **Award type:** 5
- **Project period:** 1982-08-01 → 1988-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170822

## Citation

> US National Institutes of Health, RePORTER application 3170822, TYPE 12 ADENOVIRUS TRANSFORMATION - DEFECTIVE MUTANTS (5R01CA032940-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170822. Licensed CC0.

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