# TYPE 12 ADENOVIRUS TRANSFORMATION-DEFECTIVE MUTANTS

> **NIH NIH R01** · CARNEGIE-MELLON UNIVERSITY · 1989 · $140,999

## Abstract

The overall, long-term objective of this research program is to
use genetic, immunological, and molecular approaches to define
the functions that the various type 12 adenovirus (Ad12) E1A and
E1B proteins play in transforming rat and mouse cells in vitro, and
to understand the basis for the strong oncogenicity of this
serotype and the very striking difference in viral oncogenicity
between this serotype and non-oncogenic serotypes such as 2 and
5.  We are also interested in determining the roles of these E1
gene products in regulating the progress of infection in permissive
human cells.  Our specific aims over the next five years are to:  1)
Analyze the roles of the Ad12 E1b 54Kd protein in the early phase
of productive infection in human cells, and in ocogenic
transformation of rodent cells.  2) Extend phenotypic analysis of
the Ad12 E1B 19Kd mutants with a view to determining the basis
for their differential DNA degradation and transformation
phenotypes and defining the role of this protein in productive
infection and transformation.  3) Extend mutational analysis of
the roles of Ad12 E1A gene product functions in productive
infection of human cells and oncogenic transformation of rodent
cells.  4) Prepare and characterize monoclonal antibodies specific
for each of the various Ad12 E1A and E1B proteins.

## Key facts

- **NIH application ID:** 3170823
- **Project number:** 5R01CA032940-08
- **Recipient organization:** CARNEGIE-MELLON UNIVERSITY
- **SAM.gov UEI:** U3NKNFLNQ613
- **Principal Investigator:** JAMES F WILLIAMS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $140,999
- **Award type:** 5
- **Project period:** 1982-08-01 → 1993-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170823

## Citation

> US National Institutes of Health, RePORTER application 3170823, TYPE 12 ADENOVIRUS TRANSFORMATION-DEFECTIVE MUTANTS (5R01CA032940-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170823. Licensed CC0.

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