# EBV EXPRESSION IN NASOPHARYNGEAL CARCINOMA

> **NIH NIH R01** · UNIV OF NORTH CAROLINA CHAPEL HILL · 1991 · $238,239

## Abstract

Nasopharyngeal carcinoma (NPC) is an epithelial malignancy
which develops with high incidence in Southern China, Northern Africa and
among Eskimos.  We have analyzed EBV transcription in NPC.  These studies
have revealed that the latent membrane protein (LMP) is expressed in all
NPC specimens and in the C15 NPC tumor may be encoded by two forms of LMP
mRNA.  These studies have also identified a new RNA which contains a
previously unidentified open reading frame within the BamHI A fragment.
          To further characterize EBV transcription in NPC, cDNA
libraries will be prepared to three additional tumors which have been
successfully established in nude mice.  The EBV specific cDNAs will be
identified and sequenced.  Transcription representing LMP, LMP2, and
BARFO will be analyzed in the nude mice tumors by cDNA cloning using both
oligodeoxythymidine and specific synthetic oligonucleotides for priming
of cDNA synthesis.  The polymerase chain reaction (PCR) will be utilized
to identify RNAs, containing the 171 kb intron within BARFO, identified
in C15, which is not present in a related mRNA transcribed in the B95-8
cell line.  PCR analysis will be used to identify expression of this
specific mRNA in RNA obtained from biopsy specimens and in cell lines.
To identify the authentic protein encoded by the BARFO ORF, a fusion
protein will be synthesized with glutathione in transferase.  The fusion
protein will be used to produce antisera to identify the protein as
expressed in NPC tissue.  The fusion protein will also be used to screen
human sera for reactivity to the putative protein.
          Based on our previous findings we will continue to identify
EBV-associated hyperplasias by identifying and characterizing the EBV
termini and identification of the EBERs in in situ hybridizations.

## Key facts

- **NIH application ID:** 3170876
- **Project number:** 2R01CA032979-07
- **Recipient organization:** UNIV OF NORTH CAROLINA CHAPEL HILL
- **SAM.gov UEI:** D3LHU66KBLD5
- **Principal Investigator:** NANCY JOAN RAAB-TRAUB
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $238,239
- **Award type:** 2
- **Project period:** 1983-06-01 → 1995-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170876

## Citation

> US National Institutes of Health, RePORTER application 3170876, EBV EXPRESSION IN NASOPHARYNGEAL CARCINOMA (2R01CA032979-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170876. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*