# GLYCOPROTEINS IN DIFFERENTIATION AND ONCOGENESIS

> **NIH NIH R01** · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · 1987 · $215,948

## Abstract

The overall objective of our research is to understand the course of
differentiation of human erythroid cells in terms of membrane glycoproteins
and to evaluate cell membrane glycoproteins as onco-differentiation cell
surface markers shared by tumor cells and normal cells at an early stage of
differentiation.

Two major aspects deal with:

(1)  Testing whether certain membrane glycoproteins can function as
tumor-specific markers as well as normal immature cells.  We have found
that tumor promoters diminish the expression of glycophorin on K562 cells
and HEL cells.  The results indicate that the expression of glycophorin is
closely related to the status (immature or macrophage-like cells) of cells
treated by tumor promoters.  Further studies indicate that these decreases
are due to the decrease of de novo synthesis.  Experiments are in progress
to assess whether the decrease is caused by transcriptional or
translational regulation.

(2)  Clarifying structural change of lactosaminoglycan throughout the
various ontogenic and oncogenic stages.  We have found that
lactosaminoglycan displays a drastic structural change during ontogenesis
and differentiation.  We have recently elucidated the structure of
lactosaminoglycans present in human neonatal and adult erythrocytes with
the help of a newly developed technology, fast atom bombardment mass
spectrometry.  The results revealed distinct changes in lactosaminoglycans
during development from fetal to adult erythrocytes.  Further studies will
elucidate the structures of lactosaminoglycans isolated from human
erythroleukemic cells.  (M)

## Key facts

- **NIH application ID:** 3170907
- **Project number:** 5R01CA033000-07
- **Recipient organization:** SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
- **SAM.gov UEI:** PHMKYKKJLQS1
- **Principal Investigator:** MINORU FUKUDA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $215,948
- **Award type:** 5
- **Project period:** 1982-01-01 → 1990-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170907

## Citation

> US National Institutes of Health, RePORTER application 3170907, GLYCOPROTEINS IN DIFFERENTIATION AND ONCOGENESIS (5R01CA033000-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170907. Licensed CC0.

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