# MUTATION IN HUMAN CELLS AT THE DNA SEQUENCE LEVEL

> **NIH NIH R01** · STANFORD UNIVERSITY · 1987 · $227,559

## Abstract

These experiments aim to develop systems for the analysis of mutation in
human cells at the DNA sequence level.  The approach combines the powerful
techniques of bacterial molecular genetics with recombinant DNA
methodologies.  The thoroughly studied bacterial gene lacI is the target
for mutaton and is moved in and out of human cells on vectors that can
replicate in both bacterial and mammalian cells.  After mutagenesis in
human cells, the gene is returned to E. coli, where mutations are detected
and are analyzed by rapid genetic mapping and DNA sequencing.  The system
will be used to study carcinogen-induced mutations at the sequence level
and also spontaneous mutations such as insertions of transposable
elements.  Furthermore, the experiment will be done in cell lines from
patients having hereditary diseases associated with DNA repair deficiencies
and a high frequency of cancer.  The approach should permit the study of
the molecular biology of mutation in human cells and its relation to
carcinogenesis.

## Key facts

- **NIH application ID:** 3170987
- **Project number:** 5R01CA033056-06
- **Recipient organization:** STANFORD UNIVERSITY
- **SAM.gov UEI:** HJD6G4D6TJY5
- **Principal Investigator:** MICHELE P CALOS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $227,559
- **Award type:** 5
- **Project period:** 1982-08-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170987

## Citation

> US National Institutes of Health, RePORTER application 3170987, MUTATION IN HUMAN CELLS AT THE DNA SEQUENCE LEVEL (5R01CA033056-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170987. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*