# REGULATORY FEATURES OF HSV GENE EXPRESSION

> **NIH NIH R01** · SAINT LOUIS UNIVERSITY · 1987 · $148,834

## Abstract

In this continuation we propose to continue genetic analysis of the HSV-1
cour gene that is located in the BamHI F fragment near map unit 0.645.  We
are able to assay the affects of expression of this HV-1 DNA in E. coli and
are able to select viral mutants based on the loss of induced phenotype in
E. coli after plasmid mutagenesis.  We will also study the colinear HSV-1
59K protein gene and HSV-2 61K protein gene that map near position 0.60.
The function of these proteins in the lytic cycle is unknown; however, the
expression of the carboxy terminus of the HSV-1 gene can be assayed in E.
coli.  In these experiments we will use a novel point mutant selection
technique by assay of mutated inserts aligned as alternate reading frames
in ORF vectors and combine this technique with assays of induced phenotypes
in the correct frame to select the most appropriate mutants for transfer to
the HSV-1 and HSV-2 genomes.  The mechanism of HSV-2 neoplastic
transformation by the direct repeat DNA of the mtrII will be further
investigated by the analysis of the transforming 73:2.1 extrachromosomal
element, pEX 73:2.1.  We have identified mos oncogene homology in close
association with the direct repeat DNA of the mtrII in pEX73:2.1 and mos
oncogene homology to a colinear region of the HSV-1 and HSV-2 genomes.  We
propose a structural and functional analysis of pEX73:2.1 in order to
discern the mechanism of mos activation by the direct repeat DNA and
determine the functional relationship of genes at the HSV-2 genomic mos
homology with the mtrII direct repeat DNA.

## Key facts

- **NIH application ID:** 3171052
- **Project number:** 5R01CA033101-06
- **Recipient organization:** SAINT LOUIS UNIVERSITY
- **SAM.gov UEI:** JNBLLTBTLLD8
- **Principal Investigator:** WILLIAM SM WOLD
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $148,834
- **Award type:** 5
- **Project period:** 1982-07-15 → 1988-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171052

## Citation

> US National Institutes of Health, RePORTER application 3171052, REGULATORY FEATURES OF HSV GENE EXPRESSION (5R01CA033101-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171052. Licensed CC0.

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