# SOMATIC CELL GENETICS OF T-CELL DIFFERENTIATION ANTIGENS

> **NIH NIH R01** · NEW YORK UNIVERSITY · 1988 · $93,422

## Abstract

We shall use somatic cell genetic techniques to analyze the role of Lyt2
(T8), L3T4 (T4) and Ly5 (T200) in the function and differentiation of cells
of the T lineage.  We have developed methods of producing somatic variants
of cloned normal and transformed cells, and we propose to use these
techniques to select cell lines of "normal" T cells or T-cell precursors
which either lack or express an altered form of a surface marker which
normally serves as a differentiation antigen or cell lineage marker.  We
shall also produce cell lines which egregiously express markers normally
present in other cells of the lineage or are derived from other species.
We shall clone these cells, grow them in mass culture and study their
antigenic structure, genetic organization, biological behavior and
functional properties, comparing them with both their parent line and with
immunoselected revertants.  Specifically, we plan to prepare variants of a
cultured prothymocyte line which prematurely express Lyt2 (or OKT8) and
L3T4 (or OKT4) and study their subsequent differentiation.  We will also
prepare variants of helper T cell lines which either lack L3T4, or express
either the human equivalent antigen (OKT4) or express the antigen
associated with the suppressor phenotype (OKT8).

## Key facts

- **NIH application ID:** 3171056
- **Project number:** 5R01CA033104-05
- **Recipient organization:** NEW YORK UNIVERSITY
- **SAM.gov UEI:** NX9PXMKW5KW8
- **Principal Investigator:** ROSS S BASCH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $93,422
- **Award type:** 5
- **Project period:** 1982-07-01 → 1989-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171056

## Citation

> US National Institutes of Health, RePORTER application 3171056, SOMATIC CELL GENETICS OF T-CELL DIFFERENTIATION ANTIGENS (5R01CA033104-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171056. Licensed CC0.

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