# CARCINOGENESIS OF B-LYMPHOCYTES IN RAT PEYER'S PATCHES

> **NIH NIH R01** · UNIVERSITY OF MISSISSIPPI MED CTR · 1987 · $203,704

## Abstract

The overall objective of this proposal is to study the etiology of B
lymphoid malignancies.  Using a rat model, these studies will
further address the role of the gut-associated Peyer's patches (PP)
in the chemical carcinogenesis of B lymphoid disorders.  Based on
the unique anatomical, functional and cellular characteristics of
this lymphoid organ we proposed that the lymphoid cells within
the PP 1) may be exposed to greater amounts of a potential
carcinogen which was orally introduced into the gastrointestinal
tract and 2) subsequently have a greater probability of
carcinogenesis of B-lymphocytes (the predominate cell type).
Completed studies indicate that the PP are in fact very efficient
in the adsorption of 3-methyl-cholanthrene (3-MC) from the
lumenal content.  Furthermore a high incidence of B-lymphoid
malignancies could be induced by localizing 3-MC within the PP.
Our results also indicate that the dose of the 3-MC and treatment
of the rats with pristane are important factors in the
carcinogenesis of rat lymphoid malignancies.  With high doses of
3-MC only thymic tumors were induced whereas with low doses of
3-MC plus pristane priming the B lymphoid disorders were
observed.  Two general hypotheses are proposed to address the
basis of these results.  First, exposure of the PP to low versus
high doses of 3-MC results in the generation of different 3-MC
metabolites with different cellular specificities.  And second,
pristane acts as a promoter for B lymphocytes which have
undergone an initiation event.

There are five specific aims of these studies.  First, metabolism
of low and high doses of 3-MC in PP versus other lymphoid organs,
as well as the reaction(s) of possible metabolites with lymphoid
cells will be studied.  Second, induction studies will be performed
to determine if T versus B cell malignancies can be induced by
different 3-MC metabolites.  Third, the role of pristane in the
induction of the B cell malignancies will be addressed.  Fourth,
the cellular aspects of tumoregenesis will be addressed using
transplantation methods.  Fifth, studies of the molecular
mechanisms of chemical carcinogenesis of lymphoid malignancies
will be conducted.

These studies will provide insights into the role of the Peyer's
patch in the initiation and promotion of lymphoid malignancies by
chemicals which are orally introduced into the gastrointestinal
tract.

## Key facts

- **NIH application ID:** 3171066
- **Project number:** 2R01CA033111-04A1
- **Recipient organization:** UNIVERSITY OF MISSISSIPPI MED CTR
- **SAM.gov UEI:** X59NJBFL8BJ3
- **Principal Investigator:** MARVIN A CUCHENS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $203,704
- **Award type:** 2
- **Project period:** 1983-07-01 → 1990-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171066

## Citation

> US National Institutes of Health, RePORTER application 3171066, CARCINOGENESIS OF B-LYMPHOCYTES IN RAT PEYER'S PATCHES (2R01CA033111-04A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171066. Licensed CC0.

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