# CARCINOGENESIS OF B-LYMPHOCYTES IN RAT PEYER'S PATCHES

> **NIH NIH R01** · UNIVERSITY OF MISSISSIPPI MED CTR · 1990 · $130,630

## Abstract

The overall objective of this proposal is to study the etiology of
B lymphoid malignancies.  Using a rat model, these studies will
further address the role of the gut-associated Peyer's patches (PP)
in the chemical carcinogenesis of B lymphoid disorders.  Based on
the unique anatomical, functional and cellular characteristics of
this lymphoid organ, we proposed that the lymphoid cells within the
PP 1) may be exposed to greater amounts of a potential carcinogen
which was orally introduced into the gastrointestinal tract and 2)
subsequently have a greater probability of carcinogenesis of B-
lymphocytes (the predominate cell type).  Completed studies
indicate that the PP are in fact very efficient in the adsorption
of 3-methyl-cholanthrene (3-MC) from the lumenal content.
Furthermore, a high incidence of B-lymphoid malignancies could be
induced by localizing 3-MC within the PP.  Our results also
indicate that the dose of the 3-MC and treatment of the rats with
pristane are important factors in the carcinogenesis of rat
lymphoid malignancies.  With high doses of 3-MC only thymic tumors
were induced where a with low doses of 3-MC plus pristane priming
the B lymphoid disorders were observed.  Two general hypotheses are
proposed to address the basis of these results.  First, exposure
of the PP to low versus high doses of 3-MC results in the
generation of different 3-MC metabolites with different cellular
specificities.  And second, pristane acts as a promoter for B
lymphocytes which have undergone an initiation event.

There are five specific aims of these studies.  First, metabolism
of low and high doses of 3-MC in PP versus other lymphoid organs,
as well as the reaction(s) of possible metabolites with lymphoid
cells will be studied.  Second, induction studies will be performed
to determine if T versus B cell malignancies can be induced by
different 3-MC metabolites.  Third, the role of pristane in the
induction of the B cell malignancies will be addressed.  Fourth,
the cellular aspects of tumorigenesis will be addressed using
transplantation methods.  Fifth, studies of the molecular
mechanisms of chemical carcinogenesis of lymphoid malignancies will
be conducted.

These studies will provide insights into the role of the Peyer's
patch in the initiation and promotion of lymphoid malignancies by
chemicals which are orally introduced into the gastrointestinal
tract.

## Key facts

- **NIH application ID:** 3171073
- **Project number:** 5R01CA033111-06
- **Recipient organization:** UNIVERSITY OF MISSISSIPPI MED CTR
- **SAM.gov UEI:** X59NJBFL8BJ3
- **Principal Investigator:** MARVIN A CUCHENS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $130,630
- **Award type:** 5
- **Project period:** 1983-07-01 → 1991-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171073

## Citation

> US National Institutes of Health, RePORTER application 3171073, CARCINOGENESIS OF B-LYMPHOCYTES IN RAT PEYER'S PATCHES (5R01CA033111-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171073. Licensed CC0.

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