# MULTIDIMENSIONAL SLIT-SCAN DETECTION OF BLADDER CANCER

> **NIH NIH R01** · UNIVERSITY OF ROCHESTER · 1988 · $209,189

## Abstract

The major goals of this research remain the use of quantitative
techniques to: 1) improve the sensitivity and specificity of the
detection of transitional cell carcinoma and its precursors in the
urinary bladder, 2) improve the clinical management of patients
through the generation of diagnostic and prognostic information,
and 3) improve the understanding of the biology of the various
neoplastic processes in the urinary bladder.  Multidimensional
slit-scan (MDSS) technology is used to provide quantitative
measurement or cell components.  This technology provides low
resolution morphological features together with quantitative
multiparameter fluorescence and light scatter measurements on cells
in flow.  Specific aims include:  A) Assess the sensitivity and
specificity of multiparameter MDSS flow measurements to neoplasia
through studies on bladder irrigation specimens.  This study may
provide information useful in subclassification and diagnosis; B)
Assess the prognostic significance of information derived from
propidium iodide DNA measurement, selected monoclonal markers,
and MDSS morphological features on specimens from patients
undergoing intravesical therapy for bladder cancer.  This includes
prospective longitudinal studies using bladder irrigation specimens
and retrospective longitudinal studies on paraffin embedded
material from patients with initial low grade lesions.  Features
will be correlated with treatment remission, progression and
survival; C) Evaluate a series of monoclonal antibodies supplied
through collaboration with Dr. Yves Fradet using bladder irrigation
and tissue specimens; D) Continue expansion of the Analytical
Cytology Unit bladder cancer data base.  Reduce the complex set of
clinical and flow cytometric data to a form useful in the clinical
setting; E) Continue studies on histogram comparison, analysis,
interpretation and significance; F) Develop techniques for the use
of voided urine as a specimen source through parallel microscope
based and MDSS flow studies on bladder irrigation and voided urine
specimens.  G) Continue studies in support of the goals of the
ongoing Bladder Cancer Flow Cytometry Network; H) Continue analysis
of our Acridine Orange data to guide further use of this
fluorochrome; I) Validate flow cytofluorometry specimen preparation
and staining procedures using MDSS low resolution morphological
features; and J) Improve the transportability of the slit-scan
technology by defining the minimum instrument configuration
required for generation of clinically useful features.

## Key facts

- **NIH application ID:** 3171109
- **Project number:** 2R01CA033148-07
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **SAM.gov UEI:** F27KDXZMF9Y8
- **Principal Investigator:** LEON L WHEELESS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $209,189
- **Award type:** 2
- **Project period:** 1982-08-01 → 1991-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171109

## Citation

> US National Institutes of Health, RePORTER application 3171109, MULTIDIMENSIONAL SLIT-SCAN DETECTION OF BLADDER CANCER (2R01CA033148-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171109. Licensed CC0.

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