# PROSTATE CELL SURFACE PHENOTYPE AND TUMOR BEHAVIOR

> **NIH NIH R01** · VIRGINIA COMMONWEALTH UNIVERSITY · 1988 · $5,858

## Abstract

The objective of this study is to elucidate the relationship between
expression of pathobiologically relevant surface components of human
prostatic carcinoma cells and tumor cell behavior.  The appropriate
monoclonal antibodies will be used to detect the expression of specific
cell surface components and as selective agents to enrich for cells with or
without a given surface component. A panel of human prostate carcinoma cell
clones derived from established lines, which are positive or negative with
respect to surface expression of each determinant detected by the
monoclonal antibodies, will be established.  Cell clones that vary
quantitatively in expression of each cell surface component under study
will be compared in terms of tumorigenicity, growth rate, and metastatic
behavior in athymic nude mice.  These data will be supplemented by
identification of factors that regulate expression of any prostate
tumor-related cell surface component affecting in vivo or in vitro
characteristics of the parental line.  A highly reproducible model of
spontaneous lymphatic and pulmonary metastasis by human prostate carcinoma
cells growing in nude mice has been established.  The kinetics of
metastasis, histopathology, and dose-response characteristics of this PC-3
subline have been determined, thus providing a reference system.  Thus far,
at least one less efficiently metastatic PC-3 subpopulation has been
identified and characterized in vivo and in vitro.  Treatment of PC-3
sublines with mutagens generated additional metastatic phenotypes.  Thus,
both naturally-arising and mutagen-induced phenotypes are now available for
analysis of cell surface components.  Identification of the correlation(s)
between the presence or absence of prostate tumor, cell-related,
cell-surface components and biological behavior of the cells in the nude
mouse will permit identification of specific molecular entities involved in
prostate tumor growth and metastasis.  (A)

## Key facts

- **NIH application ID:** 3171155
- **Project number:** 3R01CA033208-06S2
- **Recipient organization:** VIRGINIA COMMONWEALTH UNIVERSITY
- **SAM.gov UEI:** MLQFL4JSSAA9
- **Principal Investigator:** JOY Laurin WARE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $5,858
- **Award type:** 3
- **Project period:** 1987-07-01 → 1988-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171155

## Citation

> US National Institutes of Health, RePORTER application 3171155, PROSTATE CELL SURFACE PHENOTYPE AND TUMOR BEHAVIOR (3R01CA033208-06S2). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171155. Licensed CC0.

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