# LIPID MODULATION OF MAMMARY NEOPLASTIC GROWTH

> **NIH NIH R01** · ROSWELL PARK CANCER INSTITUTE · 1987 · $55,530

## Abstract

The major objective of this proposal is to study the promoting effect of
dietary fat on mammary carcinogenesis.  Specific aims include the
following.  (1)  The effect of quantity and type of fat on mammary
carcinogenesis induce by dimethylbenz(a) anthracene (DMBA) will be examined
in female rats.  In these experiments, saturated and unsaturated fat will
be compared with a blend of fat simulating that found in the typical
American diet.  Results of this study will be most relevant to
nutritionists and oncologists in their deliberations to recommend a
reduction of fat intake to lower the risk of breast cancer.  More
importantly, these results will specifically indicate how much the fat
content of the diet needs to be reduced or conversely, if anything
meaningful is to be gained by only a small reduction.  (2)  The efficacy of
dietary fat inpromoting mammary carcinogenesis at various times after the
administration of DMBA will be examined to determine whether rats switched
to a high fat diet several months after the administration of DMBA still
develop a high incidence of tumors.  (3)  The effect of trans fatty acids
(produced during hydrogenation in the processing of vegetable oil) on
DMBA-induced mammary carcinogenesis will be studied in female rats.  (4)
The effect of both quantity and type of dietary fat on the DNA synthetic
rate of the mammary gland will be examined by measuring the incorporation
of 3H-thymidine into the DNA of isolated epithelial cells at various times
after the administration of DMBA.  The purpose of this experiment will be
to determine if there is a correlation between the promoting effect of
dietary fat on mammary carcinogenesis and an effect of fat on the DNA
synthetic rate of the mammary gland.  (5)  The effect of quantity and type
of dietary fat will be examined in rats bearing transplantable metastasing
mammary tumors to determine if fat affects either the extent or the
location of metastases.  (6)  The effect of dietary fat on the development
and progression of ductal hyperplasia will be examined in the mammary gland
of DMBA-treated mice.  In view of the overwhelming epidmiological and
experimental evidence liking dietary fat and breast cancer, it is felt that
further research focused on defining the conditions under which fat is most
effective in stimulating mammary neoplasia should be an area of high
priority towards realizing our goal of reducing breast cancer morbidity.

## Key facts

- **NIH application ID:** 3171204
- **Project number:** 2R01CA033240-05A1
- **Recipient organization:** ROSWELL PARK CANCER INSTITUTE
- **Principal Investigator:** MARGOT M IP
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $55,530
- **Award type:** 2
- **Project period:** 1982-09-01 → 1990-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171204

## Citation

> US National Institutes of Health, RePORTER application 3171204, LIPID MODULATION OF MAMMARY NEOPLASTIC GROWTH (2R01CA033240-05A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171204. Licensed CC0.

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