# FINE CHROMOSOMAL DEFECTS IN NON-HODGKIN'S LYMPHOMA

> **NIH NIH R01** · UNIVERSITY OF MINNESOTA · 1988 · $71,483

## Abstract

Analysis of high-resolution chromosomes obtained from methotrexate (MTX)
synchronized cells of lymphomatous tissue may become an essential tool,
along with hisTopathology and immunologic cell markers, in the
subclassification of non-Hodgkin's lymphoma (NHL).  Using this technique,
we have routinely obtained mitoses at the 400 to 850 band stages and have
shown that most NHL patients can be successfully analyzed (124 of 128  = 97
percent), have an abnormal karyotype (95%), and 64 percent of them have a
specific defect that identifies distinct NHL subgroups.  Despite this high
degree of specificity, however, we have also found that each of the
non-Burkitt NHL subtypes represents a heterogeneous group of diseases and
that a specific chromosome defect may be shared among related disorders.
Such high degree of complexity may explain previous difficulties in
predicting whether NHL patients with a given histopathology and immunologic
markers will have a relatively long or short survival.  Our basic MTX
technique for lymphomatous tissue has now been further improved with the
use of bromodeoxycitidine and ethidium bromide to yield routinely 850 to
2000 chromosome bands per haploid set.  With the new technique we will
study, prospectively, approximately 500 consecutive new NHL patients to
correlate chromosomal data with response to new unified treatment protocols
from the Eastern Cooperative Oncology Group.  Our large-scale study of
consecutive patients should provide, for the first time, definitive clues
to the clinical course, response to treatment, and survival patterns of
individuals with specific primary and secondary chromosomal defects in
NHL.  It may also establish high-resolution chromosome analysis as a
critically important tool in the diagnosis, classification, prognosis, and
therapeutic assessment of this complex group of disorders.

## Key facts

- **NIH application ID:** 3171237
- **Project number:** 5R01CA033314-07
- **Recipient organization:** UNIVERSITY OF MINNESOTA
- **SAM.gov UEI:** KABJZBBJ4B54
- **Principal Investigator:** JORGE J YUNIS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $71,483
- **Award type:** 5
- **Project period:** 1982-09-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171237

## Citation

> US National Institutes of Health, RePORTER application 3171237, FINE CHROMOSOMAL DEFECTS IN NON-HODGKIN'S LYMPHOMA (5R01CA033314-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171237. Licensed CC0.

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