# MOLECULAR DETERMINANTS OF TUMORIGENICITY

> **NIH NIH R01** · UNIVERSITY OF KENTUCKY · 1987 · $123,200

## Abstract

The long-term goal of this research is to identify, at the molecular level,
those gene products that are causally related to the ability of a cell to
produce a tumor in an animal host.

Using recombinant DNA technology and a panel of cell lines (with clonal
variants) covering the range of tumorigenic potential from normal to highly
tumorigenic, the genes uniquely or abundantly expressed in the tumorigenic
cell lines will be cloned and characterized.  A selected cDNA library from
the tumorigenic cell line will be constructed and the genes governing
tumorigenicity will be identified, their mRNAs characterized, and their
protein products will be characterized and localized within the cell.  An
alternative approach is to microsequence surface antigens that have been
identified only in the more tumorigenic cell lines.  Once the sequence is
known, oligonucleotide probes can be constructed to isolate the genes'
coding for these products.

We intend to identify and characterize those products that are involved in
enhancing the tumorigenic potential of a transformed cell line, and to
initiate studies of the regulation of those genes and the role that they
play in the process of tumor formation in the host.  (I)

## Key facts

- **NIH application ID:** 3171314
- **Project number:** 5R01CA033434-05
- **Recipient organization:** UNIVERSITY OF KENTUCKY
- **SAM.gov UEI:** H1HYA8Z1NTM5
- **Principal Investigator:** Stephen G Zimmer
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $123,200
- **Award type:** 5
- **Project period:** 1982-08-01 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171314

## Citation

> US National Institutes of Health, RePORTER application 3171314, MOLECULAR DETERMINANTS OF TUMORIGENICITY (5R01CA033434-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171314. Licensed CC0.

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