# CHEMICALLY MODIFIED MONOCLONAL ANTIBODY IN TUMOR MODELS

> **NIH NIH R01** · JOHNS HOPKINS UNIVERSITY · 1987 · $253,261

## Abstract

The use of monoclonal antibodies for radioimmunoimaging and immunotherapy
will be investigated in two animal tumor systems:  a tumor virus-induced
leukemia of mice and human colonic carcinoma xenografts in hamsters.

Radioimmunoimaging of tumors with antibodies labeled with I311 will be
compared to radiometal (In111, Sc47, Ga67, and Tc99m) chelate-conjugated
antibodies.  Several derivatives including the carboxycarbonic anhydride
and the bicyclic anhydride of DTPA and the isothiocyanate of EDTA have been
prepared and will be evaluated for yield of incorporation and stability of
isotope.  Methods for chelating the radiometal prior to conjugation with
antibody will be developed to eliminate adventitious binding of isotope to
immunogloblin.  The kinetics of tumor targeting, quality of image,
catabolism, and toxicities will be evaluated.

Alpha- and beta-emitting isotypes and toxins will be coupled to
immunoglobulins for use in specific targeting of cytocidal agents to
tumors.  The therapeutic efficacy of antibodies labeled with the
beta-emitting I131 and Sc47 will be compared to the alpha-emitting Bi212.
Sequential therapy will also be evaluated.  Isotopic therapy will be
compared to immunotoxin therapy with respect to reduction of tumor burden,
prolonging survival, eradication of metastases, and toxicity.

These studies will provide a rational basis for imaging and therapy of
malignancies relevant to clinical practice.

## Key facts

- **NIH application ID:** 3171329
- **Project number:** 5R01CA033470-06
- **Recipient organization:** JOHNS HOPKINS UNIVERSITY
- **SAM.gov UEI:** FTMTDMBR29C7
- **Principal Investigator:** METTE STRAND
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $253,261
- **Award type:** 5
- **Project period:** 1982-07-01 → 1989-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171329

## Citation

> US National Institutes of Health, RePORTER application 3171329, CHEMICALLY MODIFIED MONOCLONAL ANTIBODY IN TUMOR MODELS (5R01CA033470-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171329. Licensed CC0.

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