# CELL CYCLE SPECIFIC CONTROL OF CELLULAR DIFFERENTIATION

> **NIH NIH R01** · CORNELL UNIVERSITY · 1988 · $108,173

## Abstract

These studies investigate the regulation of cellular differentiation.  The
specific primary objectives are to determine:  (1)\the amount of anteceding
proliferation needed for differentiation; (2)\the cell cycle specificity of
cellular processes regulating differentiation; and (3)\whether a central or
diverse cellular regulatory process(es) effects different courses of
differentiation in a precursor cell.  The cell system to be used is the
HL-60 human promyelocytic leukemia cell line.  Subject to different
inducers, HL-60 differentiates along either the myeloid or monocytic
pathways.  The cell-cycle kinetics and the kinetics of differentiation
effected by different inducers will be determined using multiparameter flow
cytometric analysis.  The cell-cycle specificity of regulatory processes
committing this precursor cell to myeloid or monocytic pathways of
differentiation will be determined using synchronized cells.  The primary
scientific questions addressed are: (1)\how much proliferative activity
precedes cellular differentiation; (2)\what cellular processes in the
proliferative cell cycle are implicated with a regulatory role for
effecting differentiation; and (3)\does the same or different
cellular-regulatory process(es) govern differentiation along different
pathways?  These studies directed at understanding the cellular-regulatory
mechanism of differentiation are of particular relevance to the pathology
of hematological neoplasms.  The cellular defect seminal to leukemogenesis
is attributable to a pathological differentiative process.  The studies
should increase the understanding of that pathology and hopefully promote
insight into new means of more effective clinical management of such
diseases.  Of relevance to this is the utilization of these studies of
agents which modulate cellular differentiation.  Understanding the cellular
response to such agents may lead to treatment protocols utilizing these or
related compounds.  (N)

## Key facts

- **NIH application ID:** 3171359
- **Project number:** 7R01CA033505-07
- **Recipient organization:** CORNELL UNIVERSITY
- **SAM.gov UEI:** G56PUALJ3KT5
- **Principal Investigator:** ANDREW YEN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $108,173
- **Award type:** 7
- **Project period:** 1983-08-01 → 1991-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171359

## Citation

> US National Institutes of Health, RePORTER application 3171359, CELL CYCLE SPECIFIC CONTROL OF CELLULAR DIFFERENTIATION (7R01CA033505-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171359. Licensed CC0.

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