# MECHANISMS OF REGULATION OF CELL PROLIFERATION

> **NIH NIH R01** · MAYO CLINIC ROCHESTER · 1990 · $175,082

## Abstract

The stimulation of cell proliferation by peptide growth factors is
associated with the rapid transcriptional activation of a specific set of
genes termed "competence," "immediate-early," pr "early-response" genes.
Alterations in the protein products of at least some of these genes
and/or in the mechanisms by which they are regulated are believed to be
central to both the acquisition and the progression of the transformed
phenotype.  This research will focus exclusively on the regulation of one
such gene, that encoding cytoskeletal beta-actin, and will test two major
hypotheses.  First, it is proposed that negative regulation of
transcription plays a key role in modulating beta-actin expression in
growth factor stimulated cells, and secondly, that lesions in negative
control mechanisms are responsible for altered patterns of beta-actin
expression in at least one chemically-transformed cell line.  These and
alternate possibilities will be tested by delineating cis-acting
regulatory elements and their cognate proteins which mediate the
transcriptional response of the beta-actin gene to peptide growth factors
in nontransformed AKR-2B mouse fibroblasts and in a 3-methylcholanthrene-
transformed clonal derivative.  The results of these studies will provide
insight into the complex mechanisms by which peptide growth factors
regulate specific gene expression and may provide information relevant to
proposed models for multi-stage carcinogenesis and tumor suppression.

## Key facts

- **NIH application ID:** 3171446
- **Project number:** 5R01CA033643-08
- **Recipient organization:** MAYO CLINIC ROCHESTER
- **SAM.gov UEI:** Y2K4F9RPRRG7
- **Principal Investigator:** MICHAEL J. GETZ
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $175,082
- **Award type:** 5
- **Project period:** 1983-02-01 → 1994-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171446

## Citation

> US National Institutes of Health, RePORTER application 3171446, MECHANISMS OF REGULATION OF CELL PROLIFERATION (5R01CA033643-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171446. Licensed CC0.

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