# II GLYCOLIPID BIOSYNTHESIS IN MOUSE T- AND B-LYMPHOMAS

> **NIH NIH R01** · UNIVERSITY OF NOTRE DAME · 1988 · $97,801

## Abstract

The immediate objectives of the research are fivefold:

1. Separation, characterization, and purification of G1cNAcT-2
(UDP-G1cNAc:nLcOse4Cer-(Beta1-3)-N-acetylglucosaminyltransferase) and
G1cNAcT-3 (UDP-G1cNAc:i-core(Beta1-6)N-acetylglucosaminyltransferase)
present in the neutral, detergent-solubilized super natant from p-1798
mouse T-lymphoma (estrogen-induced).

2. Purification and kinetic studies of Ga1T-4
(UDP-Gal:LcOse3Cer(Beta1-4)galactosyltransferase) from the 0.1% Triton
CF-54-solubilized supernatant fraction of p-1798 mouse T-lymphoma.

3. Completion of the biosynthesis in vitro of the I/i biantennary structure
from previously synthesized I/i core glycolipid, using purified GalT-4 and
G1cNAcT-2.

4. Characterization of FucT-2
(GDP-Fuc:nLcOse4Cer(Alpha1-2)fucosyltransferase) and FucT-3
(GDP-Fuc:LcOse3Cer(Alpha1-3)fucosyltransferase) from Beta-lymphoma (TEPC-15
alkane-induced, and ABLS-140 Abelson virus-induced).  Determination of
exact chemical linkages in the biosynthetic products
(FucAlpha-Gal-G1cNAc-Gal-G1c-Cer and FucAlpha-G1cNAc-Gal-G1c-Cer) obtained
from TEPC-15 and ABLS-140.

5. Screening of the mouse genomic library, prepared from a partial Eco RI
digestion of AJ mouse genomic DNA (Shotgun in Charon 4a) with a 32P-labeled
cDNA insert of GalT-4.  Indirect assays for the translation products for
particular GalT-4 DNA sequences, using radioiodinated monoclonal antibodies
against GalT-4.

Our overall goal is to develop an understanding of the specificities of
glycosyltransferases in mouse lymphoreticular systems using one T-lymphoma
(P-1798) and two B-lymphomas (TEPC-15 and ABLS-140) as model systems.

## Key facts

- **NIH application ID:** 3171549
- **Project number:** 5R01CA033751-06
- **Recipient organization:** UNIVERSITY OF NOTRE DAME
- **SAM.gov UEI:** FPU6XGFXMBE9
- **Principal Investigator:** MANJU BASU
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $97,801
- **Award type:** 5
- **Project period:** 1983-03-01 → 1990-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171549

## Citation

> US National Institutes of Health, RePORTER application 3171549, II GLYCOLIPID BIOSYNTHESIS IN MOUSE T- AND B-LYMPHOMAS (5R01CA033751-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171549. Licensed CC0.

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