# TERATOCARCINOMA CYTOSKELETAL PROTEINS

> **NIH NIH R01** · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · 1988 · $202,196

## Abstract

The function of intermediate filaments composed of Endo A and B proteins
will be investigated in differentiating cultures of murine embryonal
carcinoma cells and mouse embryos.  Plasmid DNAs designed to expresss an
RNA complementary to the 5' end of Endo B mRNA will be introduced into
embryonal carcinoma cells.  The effect of the expression of the antisense
RNA upon intermediate filament formation and visceral endoderm epithelium
maturation will be assessed by immunofluorescence, immunoprecipitation, and
nucleic acid hybridization techniques.  The gene coding for Endo B will be
cloned and characterized.  DNase-hypersensitive sites of the chromatin
state of the gene will be mapped and compared in embryonal carcinoma cells
and their differentiated derivatives.  Proteins which bind to specific
sequences of the Endo B gene will be identified by DNA binding assays and
chromatin footprinting experiments.  Finally, the regulatory sequences of
the Endo B gene will be identified by deletion analyses of recombinants
designed to express the chloramphenicol acetyl transferase indicator gene.
Genomic sequences will be tested for tissue-specific transcriptional
enhancer activity.

## Key facts

- **NIH application ID:** 3171694
- **Project number:** 5R01CA033946-07
- **Recipient organization:** SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
- **SAM.gov UEI:** PHMKYKKJLQS1
- **Principal Investigator:** ROBERT GLEN OSHIMA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $202,196
- **Award type:** 5
- **Project period:** 1982-06-01 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171694

## Citation

> US National Institutes of Health, RePORTER application 3171694, TERATOCARCINOMA CYTOSKELETAL PROTEINS (5R01CA033946-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171694. Licensed CC0.

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