# CELLULAR EFFECTS OF COMBINED HIGH- AND LOW-LET RADIATION

> **NIH NIH R01** · CLEVELAND CLINIC FOUNDATION · 1988 · $168,490

## Abstract

The ultimate goal of this investigation is to understand the
mechanisms underlying the biological effects of combined
treatments of low-linear-energy-transfer (LET) and high-LET
radiations.  In order to accomplish this goal, we will first study
the effects of low-LET and high-LET radiation separately to
establish their differences.  Radiation-induced mammalian DNA
single- and double-strand breaks, two of the few major DNA
lesions, and their repair kinetics will be measured and
characterized in detail.  The rates of rejoining of these breaks
inflicted by gamma-rays, fast neutrons, and their combinations
will be contrasted.  The effects on the rejoining kinetics will be
compared with cellular repair measurements, including repair of
potentially lethal damage (PLD).  Between these end points,
damage and repair at the chromosomal level will be examined.
Furthermore, single cells will be employed for exploring the
modes of cell killing in their progeny.  From these parallel
studies, the existence of eurepair and misrepair may be inferred
or identified.  Thus, the biological relevance of these processes
may be implicated.

Alkaline elution, hydroxylapatite uncoiling chromatography and
neutral elution are the techniques that will be utilized for the
DNA strand break assays.  Premature chromosome condensation
with viral fusogen will be employed for the aberration
experiments.  A semi-automated light-microscopy system is
proposed for the single cell studies.

## Key facts

- **NIH application ID:** 3171702
- **Project number:** 5R01CA033951-05
- **Recipient organization:** CLEVELAND CLINIC FOUNDATION
- **SAM.gov UEI:** TDGUJ1WA4UJ9
- **Principal Investigator:** FRANK Q. NGO
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $168,490
- **Award type:** 5
- **Project period:** 1983-07-01 → 1989-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171702

## Citation

> US National Institutes of Health, RePORTER application 3171702, CELLULAR EFFECTS OF COMBINED HIGH- AND LOW-LET RADIATION (5R01CA033951-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171702. Licensed CC0.

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