# RADIATION MUTAGENESIS IN HUMAN CELLS

> **NIH NIH R01** · HARVARD MEDICAL SCHOOL · 1988 · $119,863

## Abstract

The overall objectives of this project are to examine the mutagenic effects
of ionizing radiation in human cells.  The research proposed for the coming
grant period focuses on the study of mechanisms of radiation induced
mutations in human lymphoblastoid cells by use of cellular and molecular
probes.  The specific aims are:  1) To measure mutations at a series of
small marker loci in order to determine whether ionizing radiation produces
point mutations; 2) to develop molecular techniques to probe DNA and RNA
isolated from radiation-induced mutants at the HGPRT locus to determine if
deletions or other rearrangements have occurred; 3) to utilize these
molecular techniques to examine a collection of mutants induced by acute
x-irradiation, 125IdUrd, and low-dose-rate irradiation from tritiated
water; 4) to perform HGPRT enzyme assays on each mutant, and cytogenetic
analyses of those mutants which show molecular evidence of rearrangements;
5) to continue and extend cellular studies initiated in the previous gant
period which suggest the possibility of a nonlinear response for mutation
as a function of time of exposure to low dose rates of ionizing radiation.

Such information should enhance our knowledge of the types of DNA
modification which are responsible for the important biological effects of
ionizing radiation.  Furthermore, knowledge of the types of DNA lesions
responsible for mutagenesis may offer a scientific basis for predicting the
nature of the dose response relationship at low doses and low dose-rates.

## Key facts

- **NIH application ID:** 3171767
- **Project number:** 5R01CA034037-06
- **Recipient organization:** HARVARD MEDICAL SCHOOL
- **SAM.gov UEI:** JDLVAVGYJQ21
- **Principal Investigator:** JOHN B LITTLE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $119,863
- **Award type:** 5
- **Project period:** 1983-02-01 → 1989-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171767

## Citation

> US National Institutes of Health, RePORTER application 3171767, RADIATION MUTAGENESIS IN HUMAN CELLS (5R01CA034037-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171767. Licensed CC0.

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