# T-CELL INTERACTIONS WITH CLONED IA+ ACCESSORY CELLS

> **NIH NIH R01** · UNIVERSITY OF KENTUCKY · 1987 · $107,142

## Abstract

While MHC-restricted T cell interactions with macrophages and B
cells have been well-documented, little is known about T-T
interactions between T cell subpopulations.  During the past grant
cycle we isolated and characterized several self-Ia reactive
(autoreactive) Lyt 1+2-, L3T4+, Ia- T cell clones from normal
unimmunized DBA/2 mice and used them to analyze the
hypothesis that a T-T network existed in the normal immune
system.  These studies led to the unique observation that Lyt 1+2-
T cells isolated from normal DBA/2 mice proliferated strongly
and directly to syngeneic autoreactive T cells.  Most recently
preliminary data has indicated that normal Lyt 1+2 T cells
respond to allogeneic as well as syngeneic autoreactive T cells
suggesting that the T-T network may be "monomorphic" with
respect to class II antigens.  Based on these observations studies
will be carried out to:  1) Analyze selected characteristics of the
T-t interaction between Lyt 1+2-, L3T4+, Ia- T cells and
autoreactive T cells.  2)  Test the hypothesis that the idiotypic
markers of T cell receptors for MHC epitopes are "monomorphic"
within the species.  3) Analyze the rearrangement pattern,
expression and sequence of T cell receptor genes for auto- and
alloreactive T cell clones/hybridomas with specificity for Iad.  4)
Analyze the function of autoreactive T cells and their regulation
by Lyt 1+2-, L3T4- anti-autoreactive T cells.  These studies will
involve preparation of a series of autoreactive and anti-
autoreactive T cell hybridomas and analysis of the hybridoma
receptors by anti-autoreactive T cells and by anti-receptor
monoclonal antibodies.  The potential functional significance of
Lyt 1+2-anti-autoreactive T cells will be analyzed in vitro to
determine the modulatory effects of these cells on autoreactive T
cells.  Lastly, we will attempt to manipulate mice so as to be
able to directly access the biologic effects of autoreactive T cells
in vivo.  These experiments should provide information relative to
network regulation among T cells and its potential role in
neoplasia, autoimmunity and regulation of graft versus host
disease.

## Key facts

- **NIH application ID:** 3171788
- **Project number:** 2R01CA034052-04A1
- **Recipient organization:** UNIVERSITY OF KENTUCKY
- **SAM.gov UEI:** H1HYA8Z1NTM5
- **Principal Investigator:** ALAN KAPLAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $107,142
- **Award type:** 2
- **Project period:** 1983-06-01 → 1992-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171788

## Citation

> US National Institutes of Health, RePORTER application 3171788, T-CELL INTERACTIONS WITH CLONED IA+ ACCESSORY CELLS (2R01CA034052-04A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171788. Licensed CC0.

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