# SEQUENTIAL ANALYSIS OF ORAL CARCINOGENESIS

> **NIH NIH R01** · NORTHWESTERN UNIVERSITY · 1990 · $125,710

## Abstract

The long-range objective of the proposed research is to identify
and analyze in sequential fashion, the key cellular and molecular
events occurring and during oral carcinogenesis.  Viable cell
dissociates, full thickness whole mounts, and cryostatprepared
microscope sections, of carcinogen-treated hamster buccal pouch
epithelium (HBPE) will be examined for functional, histochemical,
immunohistochemical, and morphological evidence of cells which have
undergone one or more steps in the carcinogenic Process.  The
specific aims are:  1) to (a) further characterize a unique
keratinocyte population identified in cultures of carcinogen-
initiated HBPE, and (b) evaluate its neoplastic potential using
assays for production of transforming growth factors, in vitro
growth characteristics, angiogenesis, and tumor formation following
reimplantation into the autologous host, 2) to detect presumptive
precancerous lesions in carcinogen-initiated HBPE using monoclonal
antibodies against specific oncogene products, and 3) to determine
whether extractable constituents of smokeless tobacco exert a
promoting action on HBPE with an accompanying selection of focal
cell populations rich in gamma-glutamyl transpeptidase activity.

This research is based on the premise that a detailed analysis of
oral carcinogenesis, which focuses on the relevant participating
cell populations, will contribute to improved strategies for early
detection of precancerous oral lesions and prevention of oral
cancer.

## Key facts

- **NIH application ID:** 3171937
- **Project number:** 5R01CA034160-07
- **Recipient organization:** NORTHWESTERN UNIVERSITY
- **SAM.gov UEI:** KG76WYENL5K1
- **Principal Investigator:** Dennis B. Solt
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $125,710
- **Award type:** 5
- **Project period:** 1982-07-01 → 1990-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171937

## Citation

> US National Institutes of Health, RePORTER application 3171937, SEQUENTIAL ANALYSIS OF ORAL CARCINOGENESIS (5R01CA034160-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171937. Licensed CC0.

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