# PDGF-RECEPTOR NEGATIVE CELLS

> **NIH NIH R01** · CHILDREN'S HOSP OF PHILADELPHIA · 1990 · $196,750

## Abstract

The treatment of density-arrested BALB/c-3T3 cells with electrophoretically
homogenous or highly purified preparations of the platelet-derived growth
factor (PDGF) stimulated the rapid and selective accumulation of several
species of abundant mRNA identified by cell-free translation.  These
translatable mRNAs appeared long before entry into the S phase.  Less PDGF
was required for selective mRNA accumulation than for PDGF-modulated DNA
synthesis.  The translatable mRNAs also accumulated after addition of the
epidermal growth factor but not after addition of insulin or platelet-poor
plasma.  Their selective accumulation was blocked by addition of
actinoimycin D.  Three classes of PDGF-modulated mRNAs were defined.  An
early (primary) RNA appeared within 30 to 60 min of PDGF addition; its
accumulation was not blocked by cycloheximide.  Another early mRNA also
appeared within 60 min, but treatment with both PDGF and cycloheximide was
required for optimal accumulation.  A third class, secondary RNAs, began to
accumulate later at 90 to 120 min; the appearance of this class was
inhibited by cycloheximide.  One-\and two-dimensional gel electrophoresis
of translation products demonstrated that a spontaneously transformed
BALB/c-3T3 (ST2-3T3) cell line, which does not require PDGF or EGF for
growth, constitutively accumulated the secondary growth factor-regulated
mRNAs.  The accumulation of these translatable mRNAs may be required for
PDGF-modulated DNA synthesis.

We have recently utilized a cDNA probe of a PDGF-regulated lysosomal
protein (termed MEP) to directly quantify a PDGF-modulated mRNA.  PDGF
began to stimulate MEP mRNA accumulation 240 min after addition in a dose
dependent fashion.  Other growth factors, including EGF, IGF-1, insulin,
and platelet-poor plasma did not have this effect.  The PDGF modulated
accumulation of MEP mRNA was inhibitable by cycloheximide demonstrating
that PDGF modulated protein synthesis is required.  A spontaneously
transformed variant of BALB/c-3T3 cells which does not require PDGF for
growth accumulated MEP mRNA in a constitutive fashion.  Thus the MEP
transcript is an example of a PDGF-modulated secondary RNA.  (J)

## Key facts

- **NIH application ID:** 3171944
- **Project number:** 5R01CA034162-08
- **Recipient organization:** CHILDREN'S HOSP OF PHILADELPHIA
- **SAM.gov UEI:** G7MQPLSUX1L4
- **Principal Investigator:** CHARLES D SCHER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $196,750
- **Award type:** 5
- **Project period:** 1982-07-01 → 1990-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3171944

## Citation

> US National Institutes of Health, RePORTER application 3171944, PDGF-RECEPTOR NEGATIVE CELLS (5R01CA034162-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3171944. Licensed CC0.

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