# BIOCHEMICAL MECHANISMS OF CELLULAR INVASION

> **NIH NIH R01** · NEW YORK UNIVERSITY · 1988 · $172,882

## Abstract

The objectives of this research are:  (1)\to purify a molecule produced by
a human hepatoma cell (HHC) line which is able to induce the production of
latent collagenase and plasminogen activator (PA) in capillary endothelial
cells; and (2)\to relate the ability of this molecule to induce latent
collagenase and PA in endothelial cells in vitro to its ability to cause
neovascularization in vivo.

We have determined that human hepatoma cells produce a molecule which
stimulates by 10-\to 50-fold the production of latent collagenase and PA by
cultured, primary bovine capillary endothelial (BCE) cells.  We hypothesize
that the active molecule produced by the hepatoma cells is a physiological
inducer of neovascularization.  We believe, that during neovascularization
in vivo, this molecule stimulates the production of latent collagenase and
PA by endothelial cells, thereby inducing two enzymes required for the
penetration of new capillaries into surrounding tissues.

To test this hypothesis, we will do the following:  (1)\purify and
characterize the molecule(s) produced by HHC which induces latent
collagenase and and PA in BCE cells; (2)\assay the ability of this molecule
to induce angiogenesis in vivo; and (3)\establish cultures of human
capillary endothelial cells to determine whether the effects of the HHC
factor on human cells are comparable to those on BCE cells.  (L)

## Key facts

- **NIH application ID:** 3172005
- **Project number:** 5R01CA034282-05
- **Recipient organization:** NEW YORK UNIVERSITY
- **SAM.gov UEI:** NX9PXMKW5KW8
- **Principal Investigator:** DANIEL B RIFKIN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $172,882
- **Award type:** 5
- **Project period:** 1983-12-01 → 1988-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3172005

## Citation

> US National Institutes of Health, RePORTER application 3172005, BIOCHEMICAL MECHANISMS OF CELLULAR INVASION (5R01CA034282-05). Retrieved via MORVS 2026-10-26 from https://api.morvs.ai/grant/nih/3172005. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*