# EXPRESSION OF RETROVIRAL ENVELOPE GENE FUSION PROTEINS

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 1990 · $138,717

## Abstract

This project will study the structure and function of the gene product,
p37mos, encoded by the oncogene v-mos of Moloney murine sarcoma virus.  The
oncogene product p37mos is a member of the protein kinase family based on
amino acid sequence homology, but has not yet been shown to possess
intrinsic protein kinase activity.  We also wish  to probe the
interchangeability of cellular localization signals as regards their role
in the activation of oncogenic proteins.  The oncogenic potential of normal
cellular protein kinases which are related to the mos gene product will
also be examined.  Three areas of research are proposed.  1.)
Structure-function studies:  Site-directed mutations will be constructed in
the ATP binding site of the mos gene product, and also in other regions
defined by homology with the catalytic subunit of the cAMP dependent
protein kinase.  Linker insertion mutations will be constructed, to
identify regions in p37mos which can tolerate insertion of additional amino
acids.  Potential enzymatic activities associated with the mos gene product
will be examined using the site-directed mutants.  Potential substrate
specifications for p37mos will be studied by fusing the coding region for
kinase substrate domains to the mos coding region.  2.) Retroviral vectors
for redirecting cytological localization:  By attaching a signal sequence
for membrane translocation of the mos gene product, we have altered the
cytological localization of this oncogene product as demonstrated by
N-linked glycosylation and indirect immunofluorescence.  When anchored to
the membrane, this hybrid protein is still transforming.  Additionally, we
will examine the interchangeability of transmembrane anchor domains and
myristoylation sites for membrane attachment.  As part of this study, we
will characterize mutant v-src proteins when localized to the membrane by
means of a transmembrane anchor domain.  We will also attempt the isolation
of new transforming genes using a retroviral expression vector which
donates a signal sequence for membrane translocation.  3.) Studies of
cellular genes encoding proteins related to the mos gene product.  We
propose to randomly mutagenize normal cellular genes which encode gene
products related to the mos gene product, after which we will attempt to
isolate point mutants which are transforming.  This will allow us to
examine the oncogenic potential of these related genes.

## Key facts

- **NIH application ID:** 3172156
- **Project number:** 5R01CA034456-07
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **SAM.gov UEI:** UYTTZT6G9DT1
- **Principal Investigator:** DANIEL J DONOGHUE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $138,717
- **Award type:** 5
- **Project period:** 1983-04-01 → 1991-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3172156

## Citation

> US National Institutes of Health, RePORTER application 3172156, EXPRESSION OF RETROVIRAL ENVELOPE GENE FUSION PROTEINS (5R01CA034456-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3172156. Licensed CC0.

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