# STUDIES ON HUMAN LYMPHOCYTE ACTIVATION

> **NIH NIH R01** · UNIVERSITY OF VIRGINIA · 1989 · $208,791

## Abstract

Human T cell activation and proliferation have been probed by
monoclonal antibodies in the presence of phorbol myristate acetate
(PMA).  An early activation antigen (EA 1) which was expressed by
medullary thymocytes but not by peripheral blood T cells was
induced rapidly by PMA and synthetic diacylglycerol.  In addition,
CD28 (9.3) has been defined to mediate an activation pathway.  In
this proposal, studies are proposed to extend these findings.  Five
Aims are proposed.  (1) Because of its rapid expression and the
ease of detecting factors potentiating its expression, we wish to
define the role of monokines in the potentiation of EA 1 expression
and to identify novel monokines active on T cells.  (2) To compare
the activation requirements for EA 1 induction in T and B cells and
to relate these findings to the expression of protein kinase C
isoenzymes.  (3) To clone and to express the genes encoding the
major 22Kd deglycosylated peptide in the EA 1 complex so that the
function of EA 1 can be studied further.  (4) To further define the
CD28 pathway by comparing it to the CD3/Ti pathway.  (5) To
ascertain whether CD28 is a receptor molecule involved in cell-cell
interaction or a potential receptor for a yet to be defined
cytokine.  Parts of these studies will be carried out with CD28
isolated from cells.  To facilitate recombinant CD28 with these
studies, extracellular domains only will be generated so that
sufficient amounts of CD28 are available for detailed studies.
These studies will identify potential interactive surface molecules
important for T cell activation.  They may also provide some
insights into the monocyte related activation mechanisms, some of
which are defective in diseases with immunological deficiencies.

## Key facts

- **NIH application ID:** 3172269
- **Project number:** 5R01CA034546-09
- **Recipient organization:** UNIVERSITY OF VIRGINIA
- **SAM.gov UEI:** JJG6HU8PA4S5
- **Principal Investigator:** Shu Man Fu
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $208,791
- **Award type:** 5
- **Project period:** 1988-12-01 → 1993-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3172269

## Citation

> US National Institutes of Health, RePORTER application 3172269, STUDIES ON HUMAN LYMPHOCYTE ACTIVATION (5R01CA034546-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3172269. Licensed CC0.

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