# PRENEOPLASTIC MARKERS DETECTED BY MONOCLONAL ANTIBODIES

> **NIH NIH R01** · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · 1987 · $110,865

## Abstract

The goal of this project is to produce monoclonal antibodies to
premalignant cell populations during chemical hepatocarcinogenesis in rats
to determine whether or not expression of these phenotypes can be used to
provide a clearer understanding of carcinogenic progression and the lineage
of hepatocellular carcinoma.

We have isolated oval cells from rats after brief exposure to
n-2-acetylaminofluorene in a choline deficient diet, immunized mice, and
raised monoclonal antibodies to oval cells and normal hepatocytes.
Isolated preneoplastic cells from other carcinogenic regimens have been
used to raise additional monoclonal antibodies to oval cells, nodular
hepatocytes, and normal hepatocytes.  These monoclonal antibodies (and
additional ones to be developed) will be characterized and used to study
the epitopes on preneoplastic cells that appear during experimental models
of carcinogenesis.  These studies will delineate lineages of progression of
normal cells to cancer cells. In addition, these monoclonal antibodies have
been used to isolate specific preneoplastic cell populations from
carcinogen-treated livers by fluorescent cell sorting, and the potential
malignancy of these populations will be tested by transplantation into
syngeneic rats.  (2)

## Key facts

- **NIH application ID:** 3172401
- **Project number:** 5R01CA034635-05
- **Recipient organization:** UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
- **SAM.gov UEI:** ZUFBNVZ587D4
- **Principal Investigator:** HAROLD A DUNSFORD
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $110,865
- **Award type:** 5
- **Project period:** 1983-04-01 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3172401

## Citation

> US National Institutes of Health, RePORTER application 3172401, PRENEOPLASTIC MARKERS DETECTED BY MONOCLONAL ANTIBODIES (5R01CA034635-05). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3172401. Licensed CC0.

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