# HORMONE & RETINOID INTERACTION IN MAMMARY TISSUE

> **NIH NIH R01** · IIT RESEARCH INSTITUTE · 1990 · $134,573

## Abstract

In recent years it has been demonstrated that the growth and
differentiation of eukaryotic cells is regulated by growth factors
such as epidermal growth factor (EGF).  Retinoids on the other
hand, inhibit growth stimulatory action of EGF.  Although hormone-
retinoid interaction has been studied in the mammary tissues, no
EGF-retinoid interaction has been investigated.  In this
application we propose to investigate a three way interaction
between hormones, retinoids and EGF during mammary gland
differentiation and carcinogenesis.  Experiments will be performed
to determine (1) whether EGF has a physiological role in mammary
gland differentiation.  Effects of EGF on the development of
mammary gland, regression of the differentiated structures and
formation of nodule like alveolar lesions will be evaluated in
organ cultures; (2) whether mammary carcinogenesis is accompanied
by gradual loss of EGF receptors.  For these studies the
concentration of EGF receptors and protein kinase activity will be
measured in mammary tissues after carcinogen treatment of the
animals; studies will also be undertaken to see if retinoid can
reverse the trend; (3) whether enhancement of EGF receptor level
is required for retinoid action.  These studies will be conducted
both in cultures and in vivo; and finally, (4) whether hormones
responsible for mammary gland physiology alter EGF receptor
activity in these tissues.  These interactions will be studied in
vivo by ovariectomizing the tumor bearing animals as well as in
organ cultures by including various hormone combinations in the
medium.  Additional biochemical analyses including hormone
receptors, retinoid binding protein, and macromolecular
biosynthesis will be carried out if necessary.

## Key facts

- **NIH application ID:** 3172427
- **Project number:** 5R01CA034664-06
- **Recipient organization:** IIT RESEARCH INSTITUTE
- **SAM.gov UEI:** DGBUFQYMKWB5
- **Principal Investigator:** RAJENDRA G MEHTA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $134,573
- **Award type:** 5
- **Project period:** 1983-05-01 → 1992-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3172427

## Citation

> US National Institutes of Health, RePORTER application 3172427, HORMONE & RETINOID INTERACTION IN MAMMARY TISSUE (5R01CA034664-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3172427. Licensed CC0.

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