# CELL INTERACTIONS:  EXPRESSION OF PRENEOPLASTIC MARKERS

> **NIH NIH R01** · LOCKHEED MARTIN ENERGY SYSTEMS, INC. · 1989 · $160,872

## Abstract

We have found that when carcinogen-altered preneoplastic and neoplastic
tracheal epithelial cells (TEC) and normal TEC are cocultured in vivo in
devitalized tracheal grafts the normal cells markedly reduce the survival
of the altered cells.  A similar effect was observed in culture.  The
inhibition in culture is at least partially due to the presence of an
inhibitor present in normal TEC conditioned medium (CM).  Inhibitory TEC-CM
has been found to contain transforming growth factor type Beta.  It is not
clear, however, whether the inhibition of altered TEC by normal TEC in vivo
is associated with the production of TGF-Beta.  The proposed experiments
are intended to ultimately determine the relevance, if any, of TGF-Beta/GI
detected in TEC-CM to the control of growth and differentiation in the
intact trachea.  Similarly, we will investigate the relevance of changes in
production or sensitivity to TGF-Beta/GI observed during neoplastic
progression to the loss of growth control characteristic of neoplastic
disease.  Using combinations of in vivo, in vivo-in vitro, and in vitro
techniques developed as part of the rat tracheal cell model we will
investigate:  (1) influence of proliferation and differentiation on
TGF-Beta/GI production by normal epithelial cells; (2) effects of
carcinogen-exposure on sensitivity to inhibition by, and production of
TGF-Beta/GI; (3) changes in proliferation and differentiation induced by
TGF-Beta in sensitive target cells; (4) define cofactor requirements for
TGF-Beta/GI inhibition of TEC; and (5) determine the influence of cell
shape on TGF-Beta/GI inhibition of TEC.

## Key facts

- **NIH application ID:** 3172469
- **Project number:** 5R01CA034695-07
- **Recipient organization:** LOCKHEED MARTIN ENERGY SYSTEMS, INC.
- **Principal Investigator:** MARGARET TERZAGHI-HOWE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $160,872
- **Award type:** 5
- **Project period:** 1983-05-01 → 1991-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3172469

## Citation

> US National Institutes of Health, RePORTER application 3172469, CELL INTERACTIONS:  EXPRESSION OF PRENEOPLASTIC MARKERS (5R01CA034695-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3172469. Licensed CC0.

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