# MOLECULAR MECHANISMS IN MUSV-TS110 RNA SPLICING IN VIVO

> **NIH NIH R01** · UNIVERSITY OF TX MD ANDERSON CAN CTR · 1993 · $160,774

## Abstract

In this application we propose to continue our study of molecular
mechanisms in RNA splicing in intact cells using the novel MuSVts110
retrovirus as a model system.  As outlined in this application, our
previous work has led to an understanding of the role of intron sequences
in specifying the commitment of this pre-mRNA to splice and the unique
thermosensitivity of the splice event has allowed a promising beginning to
the study of molecular mechanisms and interactions occurring during
splicing in intact cells.  We plan to extend these studies as follows:  To
facilitate the study of pre-mRNA/spliceosome component interactions in
vivo, we plan to construct an MuSVts110 DNA whose transcription can be
controlled through the replacement of the viral enhancer with an inducible
enhancer, thus allowing us to follow preformed pre-mRNA through the
splicing pathway without interference from nascent transcripts, or to study
the splicing of nascent viral transcripts only.  Interactions between viral
transcripts and spliceosome components under splicing-permissive and
nonpermissive conditions will be monitored through the use of antibodies
specific for spliceosome components aided by techniques that allow
crosslinking of pre-mRNAs and nucleic acid moieties of spliceosome
components.  We plan to continue studies on RNA structures that specify
thermosensitive splicing.  These studies will focus on the possibility that
branchpoint positioning relative to 3' splice sites may help to regulate
MuSVts110 splicing and the inference that specific exon structure may also
be important.  In this connection, we will also determine the nature of the
changes made in the viral DNA of a series of 6m2 cell "revertants"
exhibiting stable relaxation in the growth temperature dependence of viral
RNA splicing through amplification and sequencing using the PCR technique.
Chimeric viruses will be constructed to distinguish splicing-critical
versus neutral mutations.

## Key facts

- **NIH application ID:** 3172523
- **Project number:** 5R01CA034734-10
- **Recipient organization:** UNIVERSITY OF TX MD ANDERSON CAN CTR
- **SAM.gov UEI:** S3GMKS8ELA16
- **Principal Investigator:** EDWIN C MURPHY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $160,774
- **Award type:** 5
- **Project period:** 1983-06-01 → 1994-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3172523

## Citation

> US National Institutes of Health, RePORTER application 3172523, MOLECULAR MECHANISMS IN MUSV-TS110 RNA SPLICING IN VIVO (5R01CA034734-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3172523. Licensed CC0.

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