# MAPPING CHROMOSOMES AND GENES IN RELATION TO LEUKEMIA

> **NIH NIH R01** · SLOAN-KETTERING INST CAN RESEARCH · 1993 · $217,588

## Abstract

The goal of our research continues to be the investigation of the role
played by chromosome changes in the etiology and clinical behavior of human
leukemia and lymphoma utilizing our unique tumor tissue and clinical
resources.  During the past six years of this grant we have made
significant progress and new observations in identifying new clinical,
histopathological, cytogenetic, and molecular subsets, especially of
lymphoma.  The emphasis of this application is to address a number of
specific mechanistically oriented questions deriving from our observations
of nonrandom chromosome changes and their relationship to leukemia and
lymphoma development and progression.  These are:  (1) Mechanisms of
chromosome instability and gene deregulation in lymphoma:  We will obtain
new insights into the molecular basis of chromosome breakage by sequencing
cloned translocation junctions, notably those involving the immunoglobulin
genes with c-myc, bcl-1, and ncl-2.  We will investigate c-myc deregulation
brought about by mutational changes in the exon 1/intron 1 region and its
role in the development of histologically and clinically distinct subsets
of lymphoma (follicular, diffuse large cell) carrying the t(8;14)
translocation.  For these studies we will directly sequence the target
region following in vitro amplification by the polymerase chain reaction
(PCR) technique.  (2) Genetic mechanism of lymphoma progression:  We will
investigate the role played by gene amplification in the progression to
very high grade of certain subsets of lymphoma.  For this we will clone
amplified regions from lymphomas with HSRs and DMs using the in-gel
renaturation technique.  (3) Genetic basis of leukemia onset or relapse in
response to specific treatment:  We will investigate our hypothesis that
activating ras gene mutations represent a common mechanism for the de novo
leukemogenesis that occurs in patients who have been treated for and cured
of a prior neoplasm and relapse and de novo leukemogenesis that occurs in
patients treated for leukemia by bone marrow transplantation.  The
methodology for detection of these mutations will be the PCR technique.
Finally, we will continue our productive studies of histologic correlation
and prognostic significance of chromosome changes in lymphoma.  In these
the detection of clonal chromosome abnormalities will be enhanced by the
use of molecular probes.

## Key facts

- **NIH application ID:** 3172575
- **Project number:** 5R01CA034775-10
- **Recipient organization:** SLOAN-KETTERING INST CAN RESEARCH
- **SAM.gov UEI:** KUKXRCZ6NZC2
- **Principal Investigator:** Raju S.K. Chaganti
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $217,588
- **Award type:** 5
- **Project period:** 1983-05-01 → 1994-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3172575

## Citation

> US National Institutes of Health, RePORTER application 3172575, MAPPING CHROMOSOMES AND GENES IN RELATION TO LEUKEMIA (5R01CA034775-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3172575. Licensed CC0.

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