# REGULATION OF HEMATOPOIESIS IN CANCER

> **NIH NIH R01** · INDIANA UNIVERSITY INDIANAPOLIS · 1989 · $186,080

## Abstract

The proposed project is directed towards elucidating those factors that
regulate early events in human megakaryocytic differentiation.  The
following studies will be performed:
(1) Examination of the effect of platelet microparticles present in human
serum and plasma on in vitro megakaryocytopoiesis.
(2) Examination of the effect of aplastic anemia serum on the release of
megakaryocyte colony stimulating activity by peripheral blood cells.
(3) Identification of megakaryocyte colony stimulating activity in media
conditioned by various human tissues and human continuous cell lines.
(4) Isolation and characterization of megakaryocyte colony stimulating
activity.
(5) Production of polyclonal and monoclonal antibodies to megakaryocyte
colony stimulating activity.
(6) Development of a radioimmunoassay for megakaryocyte colony stimulatilng
activity.
(7) Determination of the specificity of action of megakaryocyte colony
stimulating activity.
(8) Use of polyclonal and monoclonal antibodies to localize the site of
production of megakaryocyte colony stimulating activity immunocytochemical
techniques.
The completion of this project will result in important information
concerning both hematopoietic stem cell differentiation in general and in
specific megakaryocytic differentiation.  These studies will determine if
the stimulating activity observed in aplastic anemia serum is the result of
the presence of a regulatory molecule or merely the result of the absence
of an inhibitor.  Purification of megakaryocyte colony stimulating activity
will allow more careful studies of its biological effects and hopefully
will allow one to evaluate the use of this hematopoietic regulatory
macromolecule as a therapeutic tool in thrombocytopenic cancer patients.
The development of a radioimmunoassay for megakaryocyte colony stimulating
activity will allow us to study perturbations of this regulatory substance
in disease states.  Localization of the site of production will hopefully
result in the isolation of its cell of origin and investigation of the
regulation of the production of this factor at the cellular level.  This
information will be of use to both biologists and clinicans who are
interested in cancer biology and treatment.

## Key facts

- **NIH application ID:** 3172673
- **Project number:** 7R01CA034841-08
- **Recipient organization:** INDIANA UNIVERSITY INDIANAPOLIS
- **SAM.gov UEI:** SHHBRBAPSM35
- **Principal Investigator:** Ronald Hoffman
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $186,080
- **Award type:** 7
- **Project period:** 1982-09-01 → 1992-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3172673

## Citation

> US National Institutes of Health, RePORTER application 3172673, REGULATION OF HEMATOPOIESIS IN CANCER (7R01CA034841-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3172673. Licensed CC0.

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