# PREVENTION OF NASAL CAVITY TUMORS BY CASTRATION

> **NIH NIH R01** · UNIVERSITY OF NEBRASKA MEDICAL CENTER · 1990 · $198,677

## Abstract

Initiation of nasal and paranasal carcinogenesis by N-nitrosobis(2-
oxopropyl)amine (BOP) may be sex hormone-dependent.  Although
testosterone (T) seems the responsive hormone, a role of estrogen
(E) cannot be ruled out.  In short-term experiments the patterns
of several microsomal enzymes believed to be involved in
nitrosamine carcinogenesis, e.g., cytochromes P-450, NADPH-
cytochrome P-450 reductase, epoxide hydrolase, and glutathione X-
transferase isozymes, will be examined by immunohistochemistry in
sexually immature and mature male rats, castrated rats, and in
castrated T- or E-treated rats.  Also activities of P-450 isozymes,
particularly those with sex hormone-dependent functions, will be
investigated in nasal mucosa and livers of these rats.
Hydroxylation of radiolabeled T, benzphetamine N-demethylation, 7-
ethoxy-coumarin O-deethylation, and aniline hydroxylation will be
examined to measure enzyme activities.  The capability of nasal
mucosal homogenate to activate BOP to mutagen(s) will be studied
by the Ames and V-79 assays in rats under the above treatment
conditions.  Quantities of known BOP metabolites generated in vitro
will be assessed, and the presence of 06-methylguanine, 7-
methylguanine and 04-methylthymine in nasal mucosa of BOP-treated
rats will be examined by biochemical and immunohistochemical
methods.  1) Is there a correlation between the concentration of
sex hormones and the patterns, activities, and capabilities of
nasal mucosal enzymes to activate BOP to mutagens?  2) Are there
correlations between these enzymes and patterns of alkylated bases
and of BOP metabolites?  3) Are there correlations between
metabolizing activities, mutagenesis, alkylated bases?  4) Are
there qualitative and/or quantitative changes in the patterns
and/or activities of nasal mucosal enzymes during nasal-
carcinogenesis, and if so, can these changes be early markers for
carcinogenesis?

## Key facts

- **NIH application ID:** 3172806
- **Project number:** 5R01CA035042-06
- **Recipient organization:** UNIVERSITY OF NEBRASKA MEDICAL CENTER
- **SAM.gov UEI:** G15AG3BLLMH4
- **Principal Investigator:** PARVIZ M POUR
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $198,677
- **Award type:** 5
- **Project period:** 1984-04-01 → 1992-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3172806

## Citation

> US National Institutes of Health, RePORTER application 3172806, PREVENTION OF NASAL CAVITY TUMORS BY CASTRATION (5R01CA035042-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3172806. Licensed CC0.

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