# STRUCTURE OF CARCINOGEN-NUCLEIC ACID COMPLEXES

> **NIH NIH R01** · UNIVERSITY OF ROCHESTER · 1993 · $207,400

## Abstract

The structure of oligodeoxynucleotides modified by the environmental
carcinogens benzo(a)pyrene diol epoxide and the carcinogens
N-2-(acetylamino)fluorene and N2-(amino)fluorene will be determined by the
use of two-dimensional NMR spectroscopy. The distances obtained from NOESY
data will be used as restraints in molecular mechanics and molecular
dynamics calculations. The three dimensional structures of adducts bound to
different sequences will be determined to elucidate the effect of sequence
on the structure and dynamics of the adducts. Carcinogen-modified
oligodeoxynucleotides will be synthesized containing either codon 12 or
codon 61 sequences of the mouse and human c-Ha-ras 1 protooncogenes and the
structures determined with covalently attached carcinogens at the guanines
which are mutated in activating the oncogenes.

## Key facts

- **NIH application ID:** 3172864
- **Project number:** 5R01CA035251-10
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **SAM.gov UEI:** F27KDXZMF9Y8
- **Principal Investigator:** THOMAS R KRUGH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $207,400
- **Award type:** 5
- **Project period:** 1983-08-01 → 1995-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3172864

## Citation

> US National Institutes of Health, RePORTER application 3172864, STRUCTURE OF CARCINOGEN-NUCLEIC ACID COMPLEXES (5R01CA035251-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3172864. Licensed CC0.

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