# DNA CONTENT OF DYSPLASTIC LESIONS IN HUMAN AND RAT LIVER

> **NIH NIH R01** · CASE WESTERN RESERVE UNIVERSITY · 1987 · $142,076

## Abstract

In preliminary experiments, it has been observed by cytospectrophotometric
methods that a small number of nodules, induced in rat livers by an
induction-promotion regimen, have an aneuploid modal DNA content.  Since
aneuploidy is associated with tumor progression, it is likely that its
presence constitutes a "risk factor" for those nodules where it appears.
There is no data available on nuclear DNA content in "liver cell
dysplasia," a lesion in humans which is analogous to the rodent liver
nodule.

It is proposed to examine liver nodules induced in rats as before, using
cytospectrophotometric and histochemical methods, to determine:  a) if
aneuploidy is associated with either partial hepatectomy, initiation, or
initiation and promotion; b) the proportion of hepatomas with aneuploid
pattern generated; c) the comparative effects of "stop experiments" and
continuous promotion on aneuploid nodules; and d) the possible
correspondence between aneuploidy and persistence or remodelling of the
nodules.  It is further proposed that aneuploidy is a "risk factor" for
malignant transformation; this hypothesis will be tested by statistical
analysis of data obtained and by intrasplenic transplantation of
preneoplastic nodules.  The likelihood that human liver cell dysplasia is a
premalignant condition akin to some rat liver nodules will also be
evaluated in autopsy cases with liver cirrhosis and/or hepatoma, by
cytospectrophotometric detection of possible aneuploid modal DNA
distribution.

The experiments proposed may lead to:  a) future exploration of possible
mechanisms resulting in the aneuploid nuclear DNA content; b) less
restrictive but more veritable regulations for in vivo tests of
hepatocarcinogenesis; and c) improved prognostic value of liver biopsies,
regarding the probability of hepatoma in human patients with liver
cirrhosis.

## Key facts

- **NIH application ID:** 3172939
- **Project number:** 5R01CA035362-03
- **Recipient organization:** CASE WESTERN RESERVE UNIVERSITY
- **SAM.gov UEI:** HJMKEF7EJW69
- **Principal Investigator:** OSCAR SUDILOVSKY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $142,076
- **Award type:** 5
- **Project period:** 1985-04-01 → 1989-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3172939

## Citation

> US National Institutes of Health, RePORTER application 3172939, DNA CONTENT OF DYSPLASTIC LESIONS IN HUMAN AND RAT LIVER (5R01CA035362-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3172939. Licensed CC0.

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