# ANALYSIS OF PAPILLOMAVIRUS IN LARYNGEAL PAPILLOMATOSIS

> **NIH NIH R01** · JOHNS HOPKINS UNIVERSITY · 1987 · $117,408

## Abstract

The analysis of human papillomavirus type 6 (HPV-6) in squamous papilloma
of the larynx and respiratory tract will be continued to investigate the
molecular biology and mechanism of cellular transformation of these
viruses.  Analysis of the viral DNA in biopsy specimens has shown an
association of one subtype with a more severe disease.  These studies will
be extended to a larger population of patients to clarify the role of viral
subtypes in establishing disease.  The structural characterization of the
four subtypes of HPV-6 genomes that have been molecularly cloned from
laryngeal papillomata will be pursued by comparing the DNA sequence of
diverged regions identified among the subtypes.  Examination of DNA
sequences will reveal if base substitutions alter predicted amino acid
sequences, open reading frames, or potential splice sites that would alter
protein products and account for behavioral differences among these
viruses.  To isolate and characterize HPV-6 transcripts that have been
identified by Northern analysis and to detect low abundance mRNAs, cDNA
libraries will be constructed from RNA isolated from respiratory tract
lesions.  The size, structure and location on the viral genome of the cDNA
inserts will be determined by fine structure mapping and hybridization
analyses.  Characterized cDNA clones will be inserted into appropriate
vectors for expression in prokaryotic and eukaryotic cells to produce the
protein products of HPV-6 genomes, since understanding the mechanism of
transformation requires the identification of the protein(s) involved.
Monoclonal antibodies will be produced to nuclear proteins of laryngeal
papillomata to develop immunological probes to identify viral capsid and
non-structural proteins.  These antibodies will also be used to analyze
proteins produced by recombinants in the cDNA expression library to
establish structural/functional maps of the HPV-6 genome.  Constructs will
be made with dominant selectable markers and transfected into human primary
epithelial cells and other recipient cells to establish an in vitro model
system to investigate the oncogenic potential and biological activity of
HPV-6 DNA.  Selected cells will be assayed for expression of viral
sequences and parameters of transformation to investigate the virus-cell
interactions that are important in the replication of these human tumor
viruses.

## Key facts

- **NIH application ID:** 3173114
- **Project number:** 5R01CA035535-05
- **Recipient organization:** JOHNS HOPKINS UNIVERSITY
- **SAM.gov UEI:** FTMTDMBR29C7
- **Principal Investigator:** PHEOBE MOUNTS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $117,408
- **Award type:** 5
- **Project period:** 1983-08-01 → 1989-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173114

## Citation

> US National Institutes of Health, RePORTER application 3173114, ANALYSIS OF PAPILLOMAVIRUS IN LARYNGEAL PAPILLOMATOSIS (5R01CA035535-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173114. Licensed CC0.

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