# MOLECULAR STUDIES ON HERPES- & PAPILLOMAVIRUS PROTEINS

> **NIH NIH R01** · FRED HUTCHINSON CANCER RESEARCH CENTER · 1991 · $142,761

## Abstract

HSV-1 and HSV-2 are related viruses which have similar genomic
organization, biological properties and clinical patterns, however, they
have undergone sufficient genetic divergence to be recognized as two
distinct viruses.  It is the goal of these studies to understand the basis
of that divergence by focusing on four proteins encoded by HSV-2 and their
HSV-1 homologues.  The proteins chosen represent the full spectrum ranging
from a protein that is extremely type-specific, has been implicated in
morphological transformation, and has a molecular weight of 38,000 daltons,
to the major DNA binding protein which is highly conserved among the
herpesviruses.  Also to be studied are two proteins intermediate in their
antigenic relatedness, glycoprotein C and thymidine kinase.  To achieve
this goal we will determine the entire nucleotide sequence of these four
genes and their adjacent regulatory sequences in both HSV-1 and HSV-2.
This data will be used to predict the amino acid sequence of the proteins
and to determine the extent of nucleic acid divergence.  To measure
intratypic variation small stretches of DNA sequence will be determined
from clinical isolates of HSV-1 and HSV-2.  Using monoclonal antibodies we
will measure the humoral antibody response to these proteins in patients at
all stages of herpetic disease.  The information obtained will allow us to
generate fragments of DNA, polypeptides, and antibodies that are
type-specific and will be useful tools in assaying clinical samples.

## Key facts

- **NIH application ID:** 3173154
- **Project number:** 5R01CA035568-08
- **Recipient organization:** FRED HUTCHINSON CANCER RESEARCH CENTER
- **SAM.gov UEI:** HMSNCM57QNR5
- **Principal Investigator:** DENISE A. GALLOWAY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $142,761
- **Award type:** 5
- **Project period:** 1983-07-01 → 1991-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173154

## Citation

> US National Institutes of Health, RePORTER application 3173154, MOLECULAR STUDIES ON HERPES- & PAPILLOMAVIRUS PROTEINS (5R01CA035568-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173154. Licensed CC0.

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