# MOLECULAR PATHWAY OF HUMAN T-CELL-MEDIATED CYTOTOXICITY

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA RIVERSIDE · 1988 · $146,868

## Abstract

The objectives of this project are unchanged from the original proposal and
include in the second year:  (1) the production of monoclonal antibodies
that inhibit the in vitro cytolytic activity of human cytotoxic T-cell
lines; and (2) characterization of lymphocyte function associated antigens
(LFA) in the human cytotoxic T-cell reaction.  Mice and rats have been
immunized with longterm human cytotoxic cells and antigen preparations
derived from these cell lines, and splenocytes from such animals will be
fused with X63 myeloma cell line.  Primary screening system for identifying
monoclonal antibodies will be by inhibition of the 51Cr-release
microcytotoxicity assay and will include positive controls for blocking.
LFA-1 and T8 surface molecules will be extensively characterized for their
function in the cytolytic reaction, using monoclonal antibodies and cloned
human CTL lines.  In addition, we have studied cloned CTL lines of both the
T4 and T8 subsets which exhibit either antigen-specific or natural
killer-like activity and have shown that these cells are capable of
releasing in vitro a cytotoxic lymphokine(s).  These lymphokines exhibit
selectivity in their ability to lyse tumor cells but not normal cells.
Biochemical characterization of this cytotoxin may help to further
elucidate the mechanism(s) of how CTL lyse tumor cells.  (CS)

## Key facts

- **NIH application ID:** 3173237
- **Project number:** 5R01CA035638-05
- **Recipient organization:** UNIVERSITY OF CALIFORNIA RIVERSIDE
- **SAM.gov UEI:** MR5QC5FCAVH5
- **Principal Investigator:** Carl F Ware
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $146,868
- **Award type:** 5
- **Project period:** 1983-07-01 → 1989-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173237

## Citation

> US National Institutes of Health, RePORTER application 3173237, MOLECULAR PATHWAY OF HUMAN T-CELL-MEDIATED CYTOTOXICITY (5R01CA035638-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173237. Licensed CC0.

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