# ULTRATRACE ANALYSIS OF DNA LESIONS WITH ELECTROPHORES

> **NIH NIH R01** · NORTHEASTERN UNIVERSITY · 1987 · $145,016

## Abstract

The goal of this project is to quantitate DNA adducts in human samples by
gas chromatography with electron capture detection (GC-ECD) and with
detection by negative chemical ionization mass spectrometry (GC-NCI-MS).
High sensitivity is obtained by labeling the adducts with electrophores.
For standards of pyrimidine bases and nucleosides, detection limits at the
low fg level are currently observed.  Overall the methodology consists of
the following steps:  (1) purification of the DNA; (2) hydrolysis (acid or
enzymes) of the DNA down to DNA monomers; (3) separation of lesion (damage)
from normal DNA monomers by high performance liquid chromatography (HPLC);
and either (4a) chemical labeling of the lesion monomers with "direct
electrophores", followed by characterization and quantitation of these
electrophore-labeled monomers by GC-ECD/GC-NICI-MS, or (4b) chemical
labeling of the lesion monomers with "release tag electrophores", followed
by characterization using HPLC and quantitation by GC-ECD.  First the
methodology will be applied, including ongoing work, to several alkyl
adducts:  O6-alkyl-guanine, O4-alkyl-thymine, O2-alkyl-thymine, and
O2-alkyl-cytosine.  Next the corresponding hydroxyethyl adducts anticipated
to arise from exposure to ethylene oxide will be determined.  Finally
N6-hydroxymethyl-adenine and protein-(lysyl)-nucleoside will be determined
as potential adducts caused by exposure to formaldehyde.  This research is
needed to help determine the carcinogenic and mutagenic risks of human
exposure to chemicals.

## Key facts

- **NIH application ID:** 3173391
- **Project number:** 5R01CA035843-05
- **Recipient organization:** NORTHEASTERN UNIVERSITY
- **SAM.gov UEI:** HLTMVS2JZBS6
- **Principal Investigator:** ROGER Wallace GIESE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $145,016
- **Award type:** 5
- **Project period:** 1983-09-01 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173391

## Citation

> US National Institutes of Health, RePORTER application 3173391, ULTRATRACE ANALYSIS OF DNA LESIONS WITH ELECTROPHORES (5R01CA035843-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173391. Licensed CC0.

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