# EXPRESSION AND STRUCTURE OF INVERTEBRATE ONCOGENES

> **NIH NIH R01** · WORCESTER FOUNDATION FOR BIOMEDICAL RES · 1988 · $241,471

## Abstract

The long-term objectives of this research proposal are to define
the functional roles of Drosophila src-family oncogenes during
development.  We have concentrated our attention on two
members of this gene family:  the Drosophila src28C gene and
Drosophila epidermal growth factor receptor (DER) gene.
Recently completed analysis of the src28C cDNA sequence
revealed unique structural aspects of the encoded protein. The
protein is more basic than other src-family proteins, with a pI of
greater than 10, it has a glycine-rich amino-terminus, and a
pattern of conserved amino acids reminiscent of both tyrosine and
serine/threonine kinases.  Although the capacity of many src-
family oncogenes to cause disease when mutated has been well
established, the normal physiological roles of these genes have not
been revealed.  We will study the participation of the src-28C
gene in normal development.  The DER gene is the only example
of a potential mitotic signalling protein in Drosophila.  The
hypothetical mitotic signalling function of the Drosophila EGF
receptor homolog will be tested through the experiments
described in this proposal.  Therefore the definition of the
functional roles of these genes will contribute to knowledge of
cellular oncogene function and of Drosophila development.  The
Specific Aims of this proposal are to:  (1) Complete in situ
hybridization studies on the expression of src28c and DER RNA
during embryogenesis and metamorphosis.  (2) Isolate
temperature-sensitive mutations in the src28C and DER genes to
study their functional roles in cells and tissues where we have
documented their expression.  (3) Generate site-directed
mutations in the src28C and DER genes to study the effect of
mutations in specific regions of the proteins.  (4) Determine the
enzymatic activities of the proteins encoded by the src28C and
DER genes.

## Key facts

- **NIH application ID:** 3173435
- **Project number:** 5R01CA035911-05
- **Recipient organization:** WORCESTER FOUNDATION FOR BIOMEDICAL RES
- **Principal Investigator:** SAMUEL C WADSWORTH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $241,471
- **Award type:** 5
- **Project period:** 1984-08-01 → 1990-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173435

## Citation

> US National Institutes of Health, RePORTER application 3173435, EXPRESSION AND STRUCTURE OF INVERTEBRATE ONCOGENES (5R01CA035911-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173435. Licensed CC0.

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