# MACROPHAGE RESISTANCE VS VIRUSES AND TUMORS

> **NIH NIH R01** · ALLEGHENY UNIVERSITY OF HEALTH SCIENCES · 1988 · $40,000

## Abstract

We will continue to focus on two experimental approaches that are proving
useful in defining the origins of diversity and interrelationships that
exist among the heterogeneous-mononuclear phagocytes.  We will continue our
characterization of bone marrow-derived macrophages (BMDMO) at various
stages of growth in regard to ectoenzyme phenotypes, size distribution,
certain cell-surface markers, and resistance to herpes simplex virus
(HSV).  The intrinsic interaction of HSV with BMDMO appears to be different
from that with resident peritoneal MO (RES MO).  We are developing
methodology for molecular characterization of the HSV DNA species in
various MO populations that are infected with HSV in order to determine
whether the virus genome persists or whether abortive or defective virion
production occurs.  These studies use new methodology of dot blot
hybridization and Southern blot analysis with high specific activity HSV
DNA probes.  Our present data indicate that HSV enters resident peritoneal
MO and is uncoated, but that no viral DNA synthesis occurs.  In BMDMO, on
the other hand, some viral DNA synthesis occurs, albeit much lower than
fully permissive Vero cells, and there is complete cytopathic effect.
These differences may be related to maturational differences between these
two mononuclear phagocyte populations.

Our other experimental approach uses 89Sr to destroy bone marrow.  We are
defining the effects of this treatment on circulating, peritoneal, and
splenic cell populations in regard to MO, natural killer and natural
cytotoxic cells, and lymphocyte and polymorphonuclear leukocytes.
Determination of ectoenzyme phenotypes of various MO using the two
experimental approaches has led us to hypotheaize that the RES MO may be a
unique population.  Research in this next year will probe the mechanisms
involved in origin and maintenance of this population.  (MB)

## Key facts

- **NIH application ID:** 3173469
- **Project number:** 3R01CA035961-04S1
- **Recipient organization:** ALLEGHENY UNIVERSITY OF HEALTH SCIENCES
- **Principal Investigator:** PAGE S MORAHAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $40,000
- **Award type:** 3
- **Project period:** 1983-01-01 → 1988-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173469

## Citation

> US National Institutes of Health, RePORTER application 3173469, MACROPHAGE RESISTANCE VS VIRUSES AND TUMORS (3R01CA035961-04S1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173469. Licensed CC0.

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