# RECEPTOR-MEDIATED REGULATION OF MACROPHAGE FUNCTIONS

> **NIH NIH R01** · UNIVERSITY OF KANSAS MEDICAL CENTER · 1990 · $196,088

## Abstract

Our long-term objectives are to clearly delineate the biochemical pathways
which are triggered by the interaction between ligand and specific cell
surface receptor and lead to modulation of macrophage functions. We will
focus our efforts on the biochemical and biological properties of Fc-gamma-
Rs present on the surface of murine macrophage, because many of macrophage
functions, such as phagocytosis of opsonized particles, killing of tumor
cells, presentation of antigens, and secretion of factors which regulate T
and B cells, have been shown to require the participation of cell surface
Fc-gamma-Rs. Specific aims are to investigate: 1) physiological roles of
casein kinase II (CKII) activity associated with Fc-gamma(2a)R; 2)
properties of Fc-gamma(2a)R by biochemical and molecular biological
approaches; 3) the roles of G proteins in the regulation of phospholipases
C and A2 activated by immune complex or LPS; and 4) the role of
cAMP-dependent protein kinase (cAK) and protein kinase C (PKC) in the
regulation of macrophage functions. We will approach to these specific aims
by: 1) biochemical characterization of Fc-gamma(2a)R complex; 2) cloning of
the gene coding for Fc-gamma(2a)R by subtractive hybridization method; 3)
characterization of Fc-gamma(2a)R-associated CKII activity; 4)
investigation of Fc-gamma-R-cytoskeletal interaction during phagocytosis;
5) comparison of the nature of G proteins among macrophage cell lines; and
6) characterization of cAK and PKC. Results obtained are expected to
promote our understanding of the biochemical pathways of signal
transmittance, which are initially triggered by cell surface FC-gamma-R and
leads to the regulation of macrophage functions.

## Key facts

- **NIH application ID:** 3173486
- **Project number:** 2R01CA035977-07
- **Recipient organization:** UNIVERSITY OF KANSAS MEDICAL CENTER
- **SAM.gov UEI:** YXJGGNC5J269
- **Principal Investigator:** TSUNEO SUZUKI
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $196,088
- **Award type:** 2
- **Project period:** 1984-06-01 → 1995-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173486

## Citation

> US National Institutes of Health, RePORTER application 3173486, RECEPTOR-MEDIATED REGULATION OF MACROPHAGE FUNCTIONS (2R01CA035977-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173486. Licensed CC0.

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