# MATURATION INDUCTION OF HUMAN LEUKEMIC CELLS

> **NIH NIH R01** · NEW YORK MEDICAL COLLEGE · 1988 · $154,235

## Abstract

A physiological factor, the maturation inducer (MI, m.w. 50,000), produced
by T cells and capable of inducing the differentiation and maturation of
human myeloid leukemia cells has been described.  It has been demonstrated
to be distinct from the commonly known lymphokines and may be the human
counterpart of the murine differentiation factor MGI2 for myeloid leukemia
cells.  It has been demonstrated media in vitro and in vivo growth
suppressioin and differentiation of leukemia cells.

With the available MI molecules and specific monoclonal antibodies, we plan
to investigate the differentiation blocks and defects in leukemia, and to
evaluate differentiation indunction as a corrective approach.  The
quantitation of serum MI level in leukemia patients and normal persons will
be compared by an ELISA method using antibodies.  An assessment will be
made as to whether there is a lack of MI in patients.  Immunofluorescent
antibody techniques will be used to quantitate the MI producing
lymphocytes.  The level of producing cells in normal persons has been
determined and an assessment can be made as to whether there is a lack of
these cells in patients.  Furthermore, cell-MI interactions will be studied
by analyzing the number of membrane receptors and binding specificity.  The
effectivity of the interaction of leukemia cells with the MI will be
revealed.

Myeloid leukemia cells have been shown to be induced to differentiation
normally by the maturation inducer, suggesting the use of MI stimulation of
the production of MI in vivo, or the use of MI-producing cells as grafts.
Patient lymphocytes will be cultured with IL2 to assess MI production and
the growth of the producing cells as an autologous source.  Large
quantities of MI will be prepared by cell lines constitutively producing
MI, and by T-T cell hybridomas, using affinity chromatography, etc.  The
proposed research will reveal the basis of leukogenesis and provide crucial
information for corrective approaches.

## Key facts

- **NIH application ID:** 3173515
- **Project number:** 5R01CA035999-05
- **Recipient organization:** NEW YORK MEDICAL COLLEGE
- **SAM.gov UEI:** WK63UJ11C2H7
- **Principal Investigator:** J. W. none CHIAO
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $154,235
- **Award type:** 5
- **Project period:** 1983-03-01 → 1989-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173515

## Citation

> US National Institutes of Health, RePORTER application 3173515, MATURATION INDUCTION OF HUMAN LEUKEMIC CELLS (5R01CA035999-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173515. Licensed CC0.

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