# SV40 AND POLYOMA VIRUS TRANSFORMING PROTEINS

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 1992 · $252,799

## Abstract

Protein interactions play an important role in transformation by DNA tumor
viruses.  They might result either in activation of proto-oncogenes or, as
recently suggested, in inactivation of anti-oncogenes.  Polyoma medium T
antigen, the principal transforming protein of polyoma, binds to the
pro-oncogenes pp60c-src and pp62c-yes, thereby increasing their
protein-tyrosine kinase activity.  Medium T also binds to two cellular
proteins of 61,000 and 37,000 daltons (61K and 37K proteins), and genetic
evidence indicates that these proteins are involved in transformation.  It
is possible that they represent anti-oncogenes.  A large fraction of the
cDNA encoding the 61K protein has been sequenced, and it appears that it
belongs to a new family of proteins.  The goals of this proposal are (1) to
purify the 37K protein and to clone and sequence its corresponding cDNA, in
addition to finishing sequencing of the 61K protein cDNA; (2) to produce
large quantities of the 61K and 37K proteins; (3) to prepare polyclonal and
monoclonal antibodies against the 61K and 37K proteins; (4) to study their
expression in the cell cycle, in different organs and during development;
(5) to study the in vitro complex formation between the 61K/337K proteins
and mT antigen; (6) to microinject antibodies against the 61K/37K proteins
to test the effect of blocking complex formation in vivo on the biology of
the cell.

## Key facts

- **NIH application ID:** 3173616
- **Project number:** 5R01CA036111-08
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **SAM.gov UEI:** UYTTZT6G9DT1
- **Principal Investigator:** GERNOT F WALTER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $252,799
- **Award type:** 5
- **Project period:** 1983-12-01 → 1994-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173616

## Citation

> US National Institutes of Health, RePORTER application 3173616, SV40 AND POLYOMA VIRUS TRANSFORMING PROTEINS (5R01CA036111-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173616. Licensed CC0.

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