# HUMAN PAPILLOMA VIRUS GENE EXPRESSION

> **NIH NIH R01** · UNIVERSITY OF ROCHESTER · 1988 · $456,928

## Abstract

Human papilloma viruses (HPVs) of many genetic types cause
hyperproliferations of either cutaneous and mucosal epithelia.  Of
particular medical concern are those types associated with
venereally transmitted genital infections that can lead to benign
condylomata, dysplasias, carcinoma-in-situ and metastatic
tumors.  They cause the cytological changes for which Pap smears
screen.  The long term goals of the proposed project are to
understand the reciprocal interactions of viral and host
keratinocyte gene expression and tissue differentiation.  The
immediate ojectives are to decipher the patterns of HPV
transcription and RNA processing, the structures and biochemical
functions of the encoded proteins, and the positive and negative
regulation of the various promoters for early and late transcripts.
The proposed studies are (1) to define the transcriptional enhancer
sequences of HPV type 1; (2) to explore the molecular interactions
between the HPV-1 and -11 enhancer sequences and positive
transactivators, the E2 proteins of HPV-1 and -11, as well as a
putative negative factor, the carboxy-terminal half of the HPV-11
E2 protein (E2-C), using proteins generated from expression
vectors in E. coli and in mammalian cells; (3) to determine which
of the several promoters identified in HPV-1 and -11 warts are
responsive to the regulation of the E2 and E2-C proteins; (4) to
map the mRNA structures of HPV-16, -18 (and other prevalent
types) associated with dysplasias and carcinomas; based on these
data, mRNA  exon-specific probes will be constructed and the
viral genetic expression examined by in situ hybridization of
patient biopsies; the transcriptional patterns will be correlated
with the disease manifestation; (5) to prepare cDNA clones of
transcripts from HPV-16, -18 (and othe types) to determine how
the open reading frames (ORFs) are spliced and utilized; (6) to
express HPV-16 and -18 early region ORFs and cDNAs in E, coli
and prepare antibodies against them; (7) to detect and localize
HPV proteins in patient biopsy specimens by Western blots,
immunocytochemistry and immunoelectron microscopy; (8) to
introduce viral cDNAs and ORFs via recombinant retroviruses
into human foreskin for implantation in nude mice (the only in
vivo model system for human papilloma viruses) in order to
decipher their natural functions; and (9) to follow the infectious
cycle of human papilloma virus in human foreskin implanted in the
nude mice and the development of condylomata induced.

## Key facts

- **NIH application ID:** 3173713
- **Project number:** 5R01CA036200-05
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **SAM.gov UEI:** F27KDXZMF9Y8
- **Principal Investigator:** LOUISE T CHOW
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $456,928
- **Award type:** 5
- **Project period:** 1984-08-01 → 1992-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3173713

## Citation

> US National Institutes of Health, RePORTER application 3173713, HUMAN PAPILLOMA VIRUS GENE EXPRESSION (5R01CA036200-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3173713. Licensed CC0.

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